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Transparent tissue in solid state for solvent-free and antifade 3D imaging

Author

Listed:
  • Fu-Ting Hsiao

    (National Tsing Hua University)

  • Hung-Jen Chien

    (National Tsing Hua University)

  • Ya-Hsien Chou

    (National Tsing Hua University
    National Tsing Hua University)

  • Shih-Jung Peng

    (National Tsing Hua University)

  • Mei-Hsin Chung

    (National Tsing Hua University
    National Taiwan University Hospital-Hsinchu Branch)

  • Tzu-Hui Huang

    (National Tsing Hua University)

  • Li-Wen Lo

    (Academia Sinica)

  • Chia-Ning Shen

    (Academia Sinica
    Academia Sinica)

  • Hsiu-Pi Chang

    (National Taiwan University Hospital)

  • Chih-Yuan Lee

    (National Taiwan University Hospital)

  • Chien-Chia Chen

    (National Taiwan University Hospital)

  • Yung-Ming Jeng

    (National Taiwan University Hospital)

  • Yu-Wen Tien

    (National Taiwan University Hospital)

  • Shiue-Cheng Tang

    (National Tsing Hua University
    National Tsing Hua University
    National Tsing Hua University)

Abstract

Optical clearing with high-refractive-index (high-n) reagents is essential for 3D tissue imaging. However, the current liquid-based clearing condition and dye environment suffer from solvent evaporation and photobleaching, causing difficulties in maintaining the tissue optical and fluorescent features. Here, using the Gladstone-Dale equation [(n−1)/density=constant] as a design concept, we develop a solid (solvent-free) high-n acrylamide-based copolymer to embed mouse and human tissues for clearing and imaging. In the solid state, the fluorescent dye-labeled tissue matrices are filled and packed with the high-n copolymer, minimizing scattering in in-depth imaging and dye fading. This transparent, liquid-free condition provides a friendly tissue and cellular environment to facilitate high/super-resolution 3D imaging, preservation, transfer, and sharing among laboratories to investigate the morphologies of interest in experimental and clinical conditions.

Suggested Citation

  • Fu-Ting Hsiao & Hung-Jen Chien & Ya-Hsien Chou & Shih-Jung Peng & Mei-Hsin Chung & Tzu-Hui Huang & Li-Wen Lo & Chia-Ning Shen & Hsiu-Pi Chang & Chih-Yuan Lee & Chien-Chia Chen & Yung-Ming Jeng & Yu-We, 2023. "Transparent tissue in solid state for solvent-free and antifade 3D imaging," Nature Communications, Nature, vol. 14(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-39082-4
    DOI: 10.1038/s41467-023-39082-4
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    References listed on IDEAS

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    1. Kwanghun Chung & Jenelle Wallace & Sung-Yon Kim & Sandhiya Kalyanasundaram & Aaron S. Andalman & Thomas J. Davidson & Julie J. Mirzabekov & Kelly A. Zalocusky & Joanna Mattis & Aleksandra K. Denisin &, 2013. "Structural and molecular interrogation of intact biological systems," Nature, Nature, vol. 497(7449), pages 332-337, May.
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