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Coherent Stokes Raman scattering microscopy (CSRS)

Author

Listed:
  • Sandro Heuke

    (Institut Fresnel)

  • Hervé Rigneault

    (Institut Fresnel)

Abstract

We report the first implementation of laser scanning coherent Stokes Raman scattering (CSRS) microscopy. To overcome the major challenge in CSRS imaging, we show how to suppress the fluorescence background by narrow bandpass filter and a lock-in based demodulation. Near background free CSRS imaging of polymer beads, human skin, onion cells, avocado flesh and the wing disc of a drosphila larva are presented. Finally, we explain and demonstrate numerically that CSRS solves a major obstacle of other coherent Raman techniques by sending a significant part (up to 100%) of the CSRS photons into the backward direction under tight focusing conditions. We believe that this discovery will pave the way for numerous technological advances, e.g., in epi-detected coherent Raman multi-focus imaging, real-time laser scanning based spectroscopy or efficient endoscopy.

Suggested Citation

  • Sandro Heuke & Hervé Rigneault, 2023. "Coherent Stokes Raman scattering microscopy (CSRS)," Nature Communications, Nature, vol. 14(1), pages 1-8, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-38941-4
    DOI: 10.1038/s41467-023-38941-4
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    References listed on IDEAS

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    1. Lu Wei & Zhixing Chen & Lixue Shi & Rong Long & Andrew V. Anzalone & Luyuan Zhang & Fanghao Hu & Rafael Yuste & Virginia W. Cornish & Wei Min, 2017. "Super-multiplex vibrational imaging," Nature, Nature, vol. 544(7651), pages 465-470, April.
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