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Structures of Tetrahymena thermophila respiratory megacomplexes on the tubular mitochondrial cristae

Author

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  • Fangzhu Han

    (Zhejiang University School of Medicine
    Zhejiang University School of Medicine)

  • Yiqi Hu

    (Zhejiang University School of Medicine
    Zhejiang University School of Medicine)

  • Mengchen Wu

    (Zhejiang University School of Medicine
    Zhejiang University School of Medicine)

  • Zhaoxiang He

    (Zhejiang University School of Medicine
    Zhejiang University School of Medicine)

  • Hongtao Tian

    (Zhejiang University School of Medicine
    Zhejiang University School of Medicine)

  • Long Zhou

    (Zhejiang University School of Medicine
    Zhejiang University School of Medicine)

Abstract

Tetrahymena thermophila, a classic ciliate model organism, has been shown to possess tubular mitochondrial cristae and highly divergent electron transport chain involving four transmembrane protein complexes (I–IV). Here we report cryo-EM structures of its ~8 MDa megacomplex IV2 + (I + III2 + II)2, as well as a ~ 10.6 MDa megacomplex (IV2 + I + III2 + II)2 at lower resolution. In megacomplex IV2 + (I + III2 + II)2, each CIV2 protomer associates one copy of supercomplex I + III2 and one copy of CII, forming a half ring-shaped architecture that adapts to the membrane curvature of mitochondrial cristae. Megacomplex (IV2 + I + III2 + II)2 defines the relative position between neighbouring half rings and maintains the proximity between CIV2 and CIII2 cytochrome c binding sites. Our findings expand the current understanding of divergence in eukaryotic electron transport chain organization and how it is related to mitochondrial morphology.

Suggested Citation

  • Fangzhu Han & Yiqi Hu & Mengchen Wu & Zhaoxiang He & Hongtao Tian & Long Zhou, 2023. "Structures of Tetrahymena thermophila respiratory megacomplexes on the tubular mitochondrial cristae," Nature Communications, Nature, vol. 14(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-38158-5
    DOI: 10.1038/s41467-023-38158-5
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    References listed on IDEAS

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    1. Zhaoxiang He & Mengchen Wu & Hongtao Tian & Liangdong Wang & Yiqi Hu & Fangzhu Han & Jiancang Zhou & Yong Wang & Long Zhou, 2024. "Euglena’s atypical respiratory chain adapts to the discoidal cristae and flexible metabolism," Nature Communications, Nature, vol. 15(1), pages 1-16, December.

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