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Acoel single-cell atlas reveals expression dynamics and heterogeneity of adult pluripotent stem cells

Author

Listed:
  • Ryan E. Hulett

    (Harvard University)

  • Julian O. Kimura

    (Harvard University)

  • D. Marcela Bolaños

    (Harvard University)

  • Yi-Jyun Luo

    (Harvard University
    Academia Sinica)

  • Carlos Rivera-López

    (Harvard University
    Harvard University)

  • Lorenzo Ricci

    (Harvard University)

  • Mansi Srivastava

    (Harvard University)

Abstract

Adult pluripotent stem cell (aPSC) populations underlie whole-body regeneration in many distantly-related animal lineages, but how the underlying cellular and molecular mechanisms compare across species is unknown. Here, we apply single-cell RNA sequencing to profile transcriptional cell states of the acoel worm Hofstenia miamia during postembryonic development and regeneration. We identify cell types shared across stages and their associated gene expression dynamics during regeneration. Functional studies confirm that the aPSCs, also known as neoblasts, are the source of differentiated cells and reveal transcription factors needed for differentiation. Subclustering of neoblasts recovers transcriptionally distinct subpopulations, the majority of which are likely specialized to differentiated lineages. One neoblast subset, showing enriched expression of the histone variant H3.3, appears to lack specialization. Altogether, the cell states identified in this study facilitate comparisons to other species and enable future studies of stem cell fate potentials.

Suggested Citation

  • Ryan E. Hulett & Julian O. Kimura & D. Marcela Bolaños & Yi-Jyun Luo & Carlos Rivera-López & Lorenzo Ricci & Mansi Srivastava, 2023. "Acoel single-cell atlas reveals expression dynamics and heterogeneity of adult pluripotent stem cells," Nature Communications, Nature, vol. 14(1), pages 1-15, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-38016-4
    DOI: 10.1038/s41467-023-38016-4
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    References listed on IDEAS

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    1. Helen E. Robertson & Arnau Sebé-Pedrós & Baptiste Saudemont & Yann Loe-Mie & Anne-C. Zakrzewski & Xavier Grau-Bové & Marie-Pierre Mailhe & Philipp Schiffer & Maximilian J. Telford & Heather Marlow, 2024. "Single cell atlas of Xenoturbella bocki highlights limited cell-type complexity," Nature Communications, Nature, vol. 15(1), pages 1-11, December.
    2. Patricia Álvarez-Campos & Helena García-Castro & Elena Emili & Alberto Pérez-Posada & Irene Olmo & Sophie Peron & David A. Salamanca-Díaz & Vincent Mason & Bria Metzger & Alexandra E. Bely & Nathan J., 2024. "Annelid adult cell type diversity and their pluripotent cellular origins," Nature Communications, Nature, vol. 15(1), pages 1-22, December.

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