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Ubiquitination of CLIP-170 family protein restrains polarized growth upon DNA replication stress

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Listed:
  • Xi Wang

    (Xiamen University)

  • Fan Zheng

    (University of Science and Technology of China)

  • Yuan-yuan Yi

    (Xiamen University)

  • Gao-yuan Wang

    (Xiamen University)

  • Li-xin Hong

    (Xiamen University)

  • Dannel McCollum

    (University of Massachusetts Medical School)

  • Chuanhai Fu

    (University of Science and Technology of China)

  • Yamei Wang

    (Xiamen University)

  • Quan-wen Jin

    (Xiamen University)

Abstract

Microtubules play a crucial role during the establishment and maintenance of cell polarity. In fission yeast cells, the microtubule plus-end tracking proteins (+TIPs) (including the CLIP-170 homologue Tip1) regulate microtubule dynamics and also transport polarity factors to the cell cortex. Here, we show that the E3 ubiquitin ligase Dma1 plays an unexpected role in controlling polarized growth through ubiquitinating Tip1. Dma1 colocalizes with Tip1 to cortical sites at cell ends, and is required for ubiquitination of Tip1. Although the absence of dma1+ does not cause apparent polar growth defects in vegetatively growing cells, Dma1-mediated Tip1 ubiquitination is required to restrain polar growth upon DNA replication stress. This mechanism is distinct from the previously recognized calcineurin-dependent inhibition of polarized growth. In this work, we establish a link between Dma1-mediated Tip1 ubiquitination and DNA replication or DNA damage checkpoint-dependent inhibition of polarized growth in fission yeast.

Suggested Citation

  • Xi Wang & Fan Zheng & Yuan-yuan Yi & Gao-yuan Wang & Li-xin Hong & Dannel McCollum & Chuanhai Fu & Yamei Wang & Quan-wen Jin, 2022. "Ubiquitination of CLIP-170 family protein restrains polarized growth upon DNA replication stress," Nature Communications, Nature, vol. 13(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-33311-y
    DOI: 10.1038/s41467-022-33311-y
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    References listed on IDEAS

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    2. Hilary A. Snaith & Kenneth E. Sawin, 2003. "Fission yeast mod5p regulates polarized growth through anchoring of tea1p at cell tips," Nature, Nature, vol. 423(6940), pages 647-651, June.
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