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Three-dimensional electron ptychography of organic–inorganic hybrid nanostructures

Author

Listed:
  • Zhiyuan Ding

    (Nanjing University
    University of Oxford)

  • Si Gao

    (Nanjing University
    Nanjing Tech University)

  • Weina Fang

    (East China Normal University)

  • Chen Huang

    (University of Oxford
    Harwell Campus)

  • Liqi Zhou

    (Nanjing University)

  • Xudong Pei

    (Nanjing University)

  • Xiaoguo Liu

    (Shanghai Jiao Tong University)

  • Xiaoqing Pan

    (University of California)

  • Chunhai Fan

    (Shanghai Jiao Tong University)

  • Angus I. Kirkland

    (University of Oxford
    Harwell Campus
    Harwell Science and Innovation Campus)

  • Peng Wang

    (Nanjing University
    University of Warwick)

Abstract

Three dimensional scaffolded DNA origami with inorganic nanoparticles has been used to create tailored multidimensional nanostructures. However, the image contrast of DNA is poorer than those of the heavy nanoparticles in conventional transmission electron microscopy at high defocus so that the biological and non-biological components in 3D scaffolds cannot be simultaneously resolved using tomography of samples in a native state. We demonstrate the use of electron ptychography to recover high contrast phase information from all components in a DNA origami scaffold without staining. We further quantitatively evaluate the enhancement of contrast in comparison with conventional transmission electron microscopy. In addition, We show that for ptychography post-reconstruction focusing simplifies the workflow and reduces electron dose and beam damage.

Suggested Citation

  • Zhiyuan Ding & Si Gao & Weina Fang & Chen Huang & Liqi Zhou & Xudong Pei & Xiaoguo Liu & Xiaoqing Pan & Chunhai Fan & Angus I. Kirkland & Peng Wang, 2022. "Three-dimensional electron ptychography of organic–inorganic hybrid nanostructures," Nature Communications, Nature, vol. 13(1), pages 1-9, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-32548-x
    DOI: 10.1038/s41467-022-32548-x
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    1. Philipp M. Pelz & Sinéad M. Griffin & Scott Stonemeyer & Derek Popple & Hannah DeVyldere & Peter Ercius & Alex Zettl & Mary C. Scott & Colin Ophus, 2023. "Solving complex nanostructures with ptychographic atomic electron tomography," Nature Communications, Nature, vol. 14(1), pages 1-9, December.

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