IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v13y2022i1d10.1038_s41467-022-30131-y.html
   My bibliography  Save this article

Cooperative regulation of PBI1 and MAPKs controls WRKY45 transcription factor in rice immunity

Author

Listed:
  • Kota Ichimaru

    (Kindai University, Nakamachi)

  • Koji Yamaguchi

    (Kindai University, Nakamachi)

  • Kenichi Harada

    (Osaka University)

  • Yusaku Nishio

    (Kindai University, Nakamachi)

  • Momoka Hori

    (Kindai University, Nakamachi)

  • Kazuya Ishikawa

    (Kindai University, Nakamachi
    Iwate Biotechnology Research Center)

  • Haruhiko Inoue

    (National Agriculture and Food Research Organization (NARO))

  • Shusuke Shigeta

    (Kindai University, Nakamachi)

  • Kento Inoue

    (Kindai University, Nakamachi)

  • Keita Shimada

    (Kindai University, Nakamachi)

  • Satomi Yoshimura

    (Kindai University, Nakamachi)

  • Takumi Takeda

    (Iwate Biotechnology Research Center)

  • Eiki Yamashita

    (Osaka University)

  • Toshimichi Fujiwara

    (Osaka University)

  • Atsushi Nakagawa

    (Osaka University)

  • Chojiro Kojima

    (Osaka University
    Yokohama National University)

  • Tsutomu Kawasaki

    (Kindai University, Nakamachi
    Kindai University, Nakamachi)

Abstract

The U-box type ubiquitin ligase PUB44 positively regulates pattern-triggered immunity in rice. Here, we identify PBI1, a protein that interacts with PUB44. Crystal structure analysis indicates that PBI1 forms a four-helix bundle structure. PBI1 also interacts with WRKY45, a master transcriptional activator of rice immunity, and negatively regulates its activity. PBI1 is degraded upon perception of chitin, and this is suppressed by silencing of PUB44 or expression of XopP, indicating that PBI1 degradation depends on PUB44. These data suggest that PBI1 suppresses WRKY45 activity when cells are in an unelicited state, and during chitin signaling, PUB44-mediated degradation of PBI1 leads to activation of WRKY45. In addition, chitin-induced MAP kinase activation is required for WRKY45 activation and PBI1 degradation. These results demonstrate that chitin-induced activation of WRKY45 is regulated by the cooperation between MAP kinase-mediated phosphorylation and PUB44-mediated PBI1 degradation.

Suggested Citation

  • Kota Ichimaru & Koji Yamaguchi & Kenichi Harada & Yusaku Nishio & Momoka Hori & Kazuya Ishikawa & Haruhiko Inoue & Shusuke Shigeta & Kento Inoue & Keita Shimada & Satomi Yoshimura & Takumi Takeda & Ei, 2022. "Cooperative regulation of PBI1 and MAPKs controls WRKY45 transcription factor in rice immunity," Nature Communications, Nature, vol. 13(1), pages 1-16, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-30131-y
    DOI: 10.1038/s41467-022-30131-y
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-022-30131-y
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-022-30131-y?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Kazuya Ishikawa & Koji Yamaguchi & Kazuaki Sakamoto & Satomi Yoshimura & Kento Inoue & Seiji Tsuge & Chojiro Kojima & Tsutomu Kawasaki, 2014. "Bacterial effector modulation of host E3 ligase activity suppresses PAMP-triggered immunity in rice," Nature Communications, Nature, vol. 5(1), pages 1-11, December.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.

      More about this item

      Statistics

      Access and download statistics

      Corrections

      All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-30131-y. See general information about how to correct material in RePEc.

      If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

      If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

      If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

      For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

      Please note that corrections may take a couple of weeks to filter through the various RePEc services.

      IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.