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Bacteria eat nanoprobes for aggregation-enhanced imaging and killing diverse microorganisms

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  • Yunmin Yang

    (Suzhou Key Laboratory of Nanotechnology and Biomedicine, Institute of Functional Nano and Soft Materials (FUNSOM), and Collaborative Innovation Center of Suzhou Nano Science and Technology, Soochow University)

  • Binbin Chu

    (Suzhou Key Laboratory of Nanotechnology and Biomedicine, Institute of Functional Nano and Soft Materials (FUNSOM), and Collaborative Innovation Center of Suzhou Nano Science and Technology, Soochow University)

  • Jiayi Cheng

    (Suzhou Key Laboratory of Nanotechnology and Biomedicine, Institute of Functional Nano and Soft Materials (FUNSOM), and Collaborative Innovation Center of Suzhou Nano Science and Technology, Soochow University)

  • Jiali Tang

    (Suzhou Key Laboratory of Nanotechnology and Biomedicine, Institute of Functional Nano and Soft Materials (FUNSOM), and Collaborative Innovation Center of Suzhou Nano Science and Technology, Soochow University)

  • Bin Song

    (Suzhou Key Laboratory of Nanotechnology and Biomedicine, Institute of Functional Nano and Soft Materials (FUNSOM), and Collaborative Innovation Center of Suzhou Nano Science and Technology, Soochow University)

  • Houyu Wang

    (Suzhou Key Laboratory of Nanotechnology and Biomedicine, Institute of Functional Nano and Soft Materials (FUNSOM), and Collaborative Innovation Center of Suzhou Nano Science and Technology, Soochow University)

  • Yao He

    (Suzhou Key Laboratory of Nanotechnology and Biomedicine, Institute of Functional Nano and Soft Materials (FUNSOM), and Collaborative Innovation Center of Suzhou Nano Science and Technology, Soochow University)

Abstract

Currently optical-based techniques for in vivo microbial population imaging are limited by low imaging depth and highly light-scattering tissue; and moreover, are generally effective against only one specific group of bacteria. Here, we introduce an imaging and therapy strategy, in which different bacteria actively eat the glucose polymer (GP)-modified gold nanoparticles through ATP-binding cassette (ABC) transporter pathway, followed by laser irradiation-mediated aggregation in the bacterial cells. As a result, the aggregates display ~15.2-fold enhancement in photoacoustic signals and ~3.0-fold enhancement in antibacterial rate compared with non-aggregated counterparts. Significantly, the developed strategy allows ultrasensitive imaging of bacteria in vivo as low ~105 colony-forming unit (CFU), which is around two orders of magnitude lower than most optical contrast agents. We further demonstrate the developed strategy enables the detection of ~107 CFU bacteria residing within tumour or gut. This technique enables visualization and treatment of diverse bacteria, setting the crucial step forward the study of microbial ecosystem.

Suggested Citation

  • Yunmin Yang & Binbin Chu & Jiayi Cheng & Jiali Tang & Bin Song & Houyu Wang & Yao He, 2022. "Bacteria eat nanoprobes for aggregation-enhanced imaging and killing diverse microorganisms," Nature Communications, Nature, vol. 13(1), pages 1-15, December.
  • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-28920-6
    DOI: 10.1038/s41467-022-28920-6
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    as
    1. Claudia Errico & Juliette Pierre & Sophie Pezet & Yann Desailly & Zsolt Lenkei & Olivier Couture & Mickael Tanter, 2015. "Ultrafast ultrasound localization microscopy for deep super-resolution vascular imaging," Nature, Nature, vol. 527(7579), pages 499-502, November.
    2. Jacopo Bertolotti & Elbert G. van Putten & Christian Blum & Ad Lagendijk & Willem L. Vos & Allard P. Mosk, 2012. "Non-invasive imaging through opaque scattering layers," Nature, Nature, vol. 491(7423), pages 232-234, November.
    3. Marcelo C. Sousa, 2019. "New antibiotics target the outer membrane of bacteria," Nature, Nature, vol. 576(7787), pages 389-390, December.
    4. M. Omar Din & Tal Danino & Arthur Prindle & Matt Skalak & Jangir Selimkhanov & Kaitlin Allen & Ellixis Julio & Eta Atolia & Lev S. Tsimring & Sangeeta N. Bhatia & Jeff Hasty, 2016. "Synchronized cycles of bacterial lysis for in vivo delivery," Nature, Nature, vol. 536(7614), pages 81-85, August.
    5. Jiali Tang & Binbin Chu & Jinhua Wang & Bin Song & Yuanyuan Su & Houyu Wang & Yao He, 2019. "Multifunctional nanoagents for ultrasensitive imaging and photoactive killing of Gram-negative and Gram-positive bacteria," Nature Communications, Nature, vol. 10(1), pages 1-14, December.
    6. Aimen Zlitni & Gayatri Gowrishankar & Idan Steinberg & Tom Haywood & Sanjiv Sam Gambhir, 2020. "Maltotriose-based probes for fluorescence and photoacoustic imaging of bacterial infections," Nature Communications, Nature, vol. 11(1), pages 1-13, December.
    7. Yu Imai & Kirsten J. Meyer & Akira Iinishi & Quentin Favre-Godal & Robert Green & Sylvie Manuse & Mariaelena Caboni & Miho Mori & Samantha Niles & Meghan Ghiglieri & Chandrashekhar Honrao & Xiaoyu Ma , 2019. "A new antibiotic selectively kills Gram-negative pathogens," Nature, Nature, vol. 576(7787), pages 459-464, December.
    8. Marleen van Oosten & Tina Schäfer & Joost A. C. Gazendam & Knut Ohlsen & Eleni Tsompanidou & Marcus C. de Goffau & Hermie J. M. Harmsen & Lucia M. A. Crane & Ed Lim & Kevin P. Francis & Lael Cheung & , 2013. "Real-time in vivo imaging of invasive- and biomaterial-associated bacterial infections using fluorescently labelled vancomycin," Nature Communications, Nature, vol. 4(1), pages 1-8, December.
    9. Raymond W. Bourdeau & Audrey Lee-Gosselin & Anupama Lakshmanan & Arash Farhadi & Sripriya Ravindra Kumar & Suchita P. Nety & Mikhail G. Shapiro, 2018. "Acoustic reporter genes for noninvasive imaging of microorganisms in mammalian hosts," Nature, Nature, vol. 553(7686), pages 86-90, January.
    10. Gregory D. Poore & Evguenia Kopylova & Qiyun Zhu & Carolina Carpenter & Serena Fraraccio & Stephen Wandro & Tomasz Kosciolek & Stefan Janssen & Jessica Metcalf & Se Jin Song & Jad Kanbar & Sandrine Mi, 2020. "RETRACTED ARTICLE: Microbiome analyses of blood and tissues suggest cancer diagnostic approach," Nature, Nature, vol. 579(7800), pages 567-574, March.
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    1. Qian Zhang & Bin Song & Yanan Xu & Yunmin Yang & Jian Ji & Wenjun Cao & Jianping Lu & Jiali Ding & Haiting Cao & Binbin Chu & Jiaxu Hong & Houyu Wang & Yao He, 2023. "In vivo bioluminescence imaging of natural bacteria within deep tissues via ATP-binding cassette sugar transporter," Nature Communications, Nature, vol. 14(1), pages 1-14, December.
    2. Zehui Wang & Anhua Wu & Wen Cheng & Yuhe Li & Dingxuan Li & Lai Wang & Xinfu Zhang & Yi Xiao, 2023. "Adoptive macrophage directed photodynamic therapy of multidrug-resistant bacterial infection," Nature Communications, Nature, vol. 14(1), pages 1-16, December.

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