IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v12y2021i1d10.1038_s41467-021-26462-x.html
   My bibliography  Save this article

Drosophila ßHeavy-Spectrin is required in polarized ensheathing glia that form a diffusion-barrier around the neuropil

Author

Listed:
  • Nicole Pogodalla

    (Universität Münster)

  • Holger Kranenburg

    (Universität Münster)

  • Simone Rey

    (Universität Münster)

  • Silke Rodrigues

    (Universität Münster)

  • Albert Cardona

    (Cambridge Biomedical Campus
    University of Cambridge
    HHMI Janelia Research Campus)

  • Christian Klämbt

    (Universität Münster)

Abstract

In the central nervous system (CNS), functional tasks are often allocated to distinct compartments. This is also evident in the Drosophila CNS where synapses and dendrites are clustered in distinct neuropil regions. The neuropil is separated from neuronal cell bodies by ensheathing glia, which as we show using dye injection experiments, contribute to the formation of an internal diffusion barrier. We find that ensheathing glia are polarized with a basolateral plasma membrane rich in phosphatidylinositol-(3,4,5)-triphosphate (PIP3) and the Na+/K+-ATPase Nervana2 (Nrv2) that abuts an extracellular matrix formed at neuropil-cortex interface. The apical plasma membrane is facing the neuropil and is rich in phosphatidylinositol-(4,5)-bisphosphate (PIP2) that is supported by a sub-membranous ßHeavy-Spectrin cytoskeleton. ßHeavy-spectrin mutant larvae affect ensheathing glial cell polarity with delocalized PIP2 and Nrv2 and exhibit an abnormal locomotion which is similarly shown by ensheathing glia ablated larvae. Thus, polarized glia compartmentalizes the brain and is essential for proper nervous system function.

Suggested Citation

  • Nicole Pogodalla & Holger Kranenburg & Simone Rey & Silke Rodrigues & Albert Cardona & Christian Klämbt, 2021. "Drosophila ßHeavy-Spectrin is required in polarized ensheathing glia that form a diffusion-barrier around the neuropil," Nature Communications, Nature, vol. 12(1), pages 1-18, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-26462-x
    DOI: 10.1038/s41467-021-26462-x
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-021-26462-x
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-021-26462-x?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Zhiguo Ma & Tobias Stork & Dwight E. Bergles & Marc R. Freeman, 2016. "Neuromodulators signal through astrocytes to alter neural circuit activity and behaviour," Nature, Nature, vol. 539(7629), pages 428-432, November.
    2. Tomoko Ohyama & Casey M. Schneider-Mizell & Richard D. Fetter & Javier Valdes Aleman & Romain Franconville & Marta Rivera-Alba & Brett D. Mensh & Kristin M. Branson & Julie H. Simpson & James W. Truma, 2015. "A multilevel multimodal circuit enhances action selection in Drosophila," Nature, Nature, vol. 520(7549), pages 633-639, April.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Máté Fellner & Bálint Varga & Vince Grolmusz, 2020. "The frequent complete subgraphs in the human connectome," PLOS ONE, Public Library of Science, vol. 15(8), pages 1-12, August.
    2. Mami Nakamizo-Dojo & Kenichi Ishii & Jiro Yoshino & Masato Tsuji & Kazuo Emoto, 2023. "Descending GABAergic pathway links brain sugar-sensing to peripheral nociceptive gating in Drosophila," Nature Communications, Nature, vol. 14(1), pages 1-18, December.
    3. Valentina Botero & Bethany A. Stanhope & Elizabeth B. Brown & Eliza C. Grenci & Tamara Boto & Scarlet J. Park & Lanikea B. King & Keith R. Murphy & Kenneth J. Colodner & James A. Walker & Alex C. Keen, 2021. "Neurofibromin regulates metabolic rate via neuronal mechanisms in Drosophila," Nature Communications, Nature, vol. 12(1), pages 1-13, December.
    4. Lan Pang & Zhiguo Liu & Jiani Chen & Zhi Dong & Sicong Zhou & Qichao Zhang & Yueqi Lu & Yifeng Sheng & Xuexin Chen & Jianhua Huang, 2022. "Search performance and octopamine neuronal signaling mediate parasitoid induced changes in Drosophila oviposition behavior," Nature Communications, Nature, vol. 13(1), pages 1-18, December.
    5. Fushun Wang & Wei Wang & Simeng Gu & Dan Qi & Nathan A. Smith & Weiguo Peng & Wei Dong & Jiajin Yuan & Binbin Zhao & Ying Mao & Peng Cao & Qing Richard Lu & Lee A. Shapiro & S. Stephen Yi & Erxi Wu & , 2023. "Distinct astrocytic modulatory roles in sensory transmission during sleep, wakefulness, and arousal states in freely moving mice," Nature Communications, Nature, vol. 14(1), pages 1-12, December.
    6. Stefano Recanatesi & Gabriel Koch Ocker & Michael A Buice & Eric Shea-Brown, 2019. "Dimensionality in recurrent spiking networks: Global trends in activity and local origins in connectivity," PLOS Computational Biology, Public Library of Science, vol. 15(7), pages 1-29, July.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-26462-x. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.