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High-resolution label-free 3D mapping of extracellular pH of single living cells

Author

Listed:
  • Yanjun Zhang

    (Imperial College London
    Tianjin Medical University General Hospital)

  • Yasufumi Takahashi

    (Kanazawa University, Kakuma-machi
    Japan Science and Technology Agency (JST))

  • Sung Pil Hong

    (Imperial College London)

  • Fengjie Liu

    (Department of Earth Science & Engineering, Imperial College London)

  • Joanna Bednarska

    (Imperial College London)

  • Philip S. Goff

    (St George’s, University of London)

  • Pavel Novak

    (Imperial College London
    National University of Science and Technology “MISIS”)

  • Andrew Shevchuk

    (Imperial College London)

  • Sahana Gopal

    (Imperial College London)

  • Iros Barozzi

    (Imperial College London)

  • Luca Magnani

    (Imperial College London)

  • Hideki Sakai

    (University of Toyama)

  • Yoshimoto Suguru

    (Kanazawa University, Kakuma-machi)

  • Takuto Fujii

    (University of Toyama)

  • Alexander Erofeev

    (National University of Science and Technology “MISIS”
    Lomonosov Moscow State University)

  • Peter Gorelkin

    (National University of Science and Technology “MISIS”)

  • Alexander Majouga

    (Lomonosov Moscow State University)

  • Dominik J. Weiss

    (Department of Earth Science & Engineering, Imperial College London)

  • Christopher Edwards

    (Imperial College London)

  • Aleksandar P. Ivanov

    (Imperial College London)

  • David Klenerman

    (University of Cambridge)

  • Elena V. Sviderskaya

    (St George’s, University of London)

  • Joshua B. Edel

    (Imperial College London)

  • Yuri Korchev

    (Imperial College London
    Kanazawa University, Kakuma-machi)

Abstract

Dynamic mapping of extracellular pH (pHe) at the single-cell level is critical for understanding the role of H+ in cellular and subcellular processes, with particular importance in cancer. While several pHe sensing techniques have been developed, accessing this information at the single-cell level requires improvement in sensitivity, spatial and temporal resolution. We report on a zwitterionic label-free pH nanoprobe that addresses these long-standing challenges. The probe has a sensitivity > 0.01 units, 2 ms response time, and 50 nm spatial resolution. The platform was integrated into a double-barrel nanoprobe combining pH sensing with feedback-controlled distance dependance via Scanning Ion Conductance Microscopy. This allows for the simultaneous 3D topographical imaging and pHe monitoring of living cancer cells. These classes of nanoprobes were used for real-time high spatiotemporal resolution pHe mapping at the subcellular level and revealed tumour heterogeneity of the peri-cellular environments of melanoma and breast cancer cells.

Suggested Citation

  • Yanjun Zhang & Yasufumi Takahashi & Sung Pil Hong & Fengjie Liu & Joanna Bednarska & Philip S. Goff & Pavel Novak & Andrew Shevchuk & Sahana Gopal & Iros Barozzi & Luca Magnani & Hideki Sakai & Yoshim, 2019. "High-resolution label-free 3D mapping of extracellular pH of single living cells," Nature Communications, Nature, vol. 10(1), pages 1-9, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-13535-1
    DOI: 10.1038/s41467-019-13535-1
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