IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v10y2019i1d10.1038_s41467-019-13153-x.html
   My bibliography  Save this article

Packaging and delivering enzymes by amorphous metal-organic frameworks

Author

Listed:
  • Xiaoling Wu

    (Tsinghua University
    South China University of Technology)

  • Hua Yue

    (Chinese Academy of Sciences)

  • Yuanyu Zhang

    (Tsinghua University)

  • Xiaoyong Gao

    (Chinese Academy of Sciences)

  • Xiaoyang Li

    (Tsinghua University)

  • Licheng Wang

    (Tsinghua University)

  • Yufei Cao

    (Tsinghua University)

  • Miao Hou

    (Tsinghua University)

  • Haixia An

    (Chinese Academy of Sciences)

  • Lin Zhang

    (Tianjin University)

  • Sai Li

    (Tsinghua University
    Tsinghua-Peking Joint Center for Life Sciences
    Beijing Advanced Innovation Center for Structural Biology)

  • Jingyuan Ma

    (Chinese Academy of Sciences)

  • He Lin

    (Chinese Academy of Sciences)

  • Yanan Fu

    (Chinese Academy of Sciences)

  • Hongkai Gu

    (Jilin University)

  • Wenyong Lou

    (South China University of Technology)

  • Wei Wei

    (Chinese Academy of Sciences)

  • Richard N. Zare

    (Fudan University, Jiangwan Campus)

  • Jun Ge

    (Tsinghua University
    Tsinghua Shenzhen International Graduate School)

Abstract

Enzymatic catalysis in living cells enables the in-situ detection of cellular metabolites in single cells, which could contribute to early diagnosis of diseases. In this study, enzyme is packaged in amorphous metal-organic frameworks (MOFs) via a one-pot co-precipitation process under ambient conditions, exhibiting 5–20 times higher apparent activity than when the enzyme is encapsulated in corresponding crystalline MOFs. Molecular simulation and cryo-electron tomography (Cryo-ET) combined with other techniques demonstrate that the mesopores generated in this disordered and fuzzy structure endow the packaged enzyme with high enzyme activity. The highly active glucose oxidase delivered by the amorphous MOF nanoparticles allows the noninvasive and facile measurement of glucose in single living cells, which can be used to distinguish between cancerous and normal cells.

Suggested Citation

  • Xiaoling Wu & Hua Yue & Yuanyu Zhang & Xiaoyong Gao & Xiaoyang Li & Licheng Wang & Yufei Cao & Miao Hou & Haixia An & Lin Zhang & Sai Li & Jingyuan Ma & He Lin & Yanan Fu & Hongkai Gu & Wenyong Lou & , 2019. "Packaging and delivering enzymes by amorphous metal-organic frameworks," Nature Communications, Nature, vol. 10(1), pages 1-8, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-13153-x
    DOI: 10.1038/s41467-019-13153-x
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-019-13153-x
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-019-13153-x?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Wei Huang & Haitao Yuan & Huangsheng Yang & Xiaomin Ma & Shuyao Huang & Hongjie Zhang & Siming Huang & Guosheng Chen & Gangfeng Ouyang, 2023. "Green synthesis of stable hybrid biocatalyst using a hydrogen-bonded, π-π-stacking supramolecular assembly for electrochemical immunosensor," Nature Communications, Nature, vol. 14(1), pages 1-11, December.
    2. Linjing Tong & Siming Huang & Yujian Shen & Suya Liu & Xiaomin Ma & Fang Zhu & Guosheng Chen & Gangfeng Ouyang, 2022. "Atomically unveiling the structure-activity relationship of biomacromolecule-metal-organic frameworks symbiotic crystal," Nature Communications, Nature, vol. 13(1), pages 1-9, December.
    3. Sassone, Daniele & Bocchini, Sergio & Fontana, Marco & Salvini, Clara & Cicero, Giancarlo & Re Fiorentin, Michele & Risplendi, Francesca & Latini, Giulio & Amin Farkhondehfal, M. & Pirri, Fabrizio & Z, 2022. "Imidazole-imidazolate pair as organo-electrocatalyst for CO2 reduction on ZIF-8 material," Applied Energy, Elsevier, vol. 324(C).
    4. Ke Li & Yucheng Zhao & Jian Yang & Jinlou Gu, 2022. "Nanoemulsion-directed growth of MOFs with versatile architectures for the heterogeneous regeneration of coenzymes," Nature Communications, Nature, vol. 13(1), pages 1-8, December.
    5. Danping Tian & Ruipeng Hao & Xiaoming Zhang & Hu Shi & Yuwei Wang & Linfeng Liang & Haichao Liu & Hengquan Yang, 2023. "Multi-compartmental MOF microreactors derived from Pickering double emulsions for chemo-enzymatic cascade catalysis," Nature Communications, Nature, vol. 14(1), pages 1-14, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-13153-x. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.