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Metal-Tolerant Bacteria of Wastewater Treatment Plant in a Large City

Author

Listed:
  • Leonid Perelomov

    (Laboratory of Biogeochemistry, Tula State Lev Tolstoy Pedagogical University, Lenin Avenue, 125, 300026 Tula, Russia)

  • Olga Sizova

    (Federal Research Center “Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences”, G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms of RAS, 142290 Pushchino, Russia)

  • Mohammad Mahmudur Rahman

    (Global Centre for Environmental Remediation (GCER), College of Engineering, Science and Environment, The University of Newcastle, Callaghan, NSW 2308, Australia
    Department of General Educational Development, Faculty of Science & Information Technology, Daffodil International University, Dhaka 1207, Bangladesh)

  • Irina Perelomova

    (Medical Institute, Tula State University, Lenin Avenue, 92, 300012 Tula, Russia)

  • Tatiana Minkina

    (Academy of Biology and Biotechnology, Southern Federal University, 344006 Rostov-on-Don, Russia)

  • Sergei Sokolov

    (Federal Research Center “Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences”, G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms of RAS, 142290 Pushchino, Russia)

  • Yury Atroshchenko

    (Center for Technology Excellence, Tula State Lev Tolstoy Pedagogical University, Lenin Avenue, 125, 300026 Tula, Russia)

Abstract

Biological treatment methods are the most important part of the treatment process for domestic wastewater, the amounts of which are increasing every year due to rapid, unregulated urbanization and the rising number of people living in such areas. At the same time, microorganisms existing in treatment facilities should not only effectively utilize organic pollutants, but also be resistant to a variety of organic and inorganic contaminants. This study’s objective is to isolate and identify—using molecular genetic techniques—strains of bacteria that tolerate concentrations of heavy metals (Ni, Cd, Pb, Zn and Cu) in the 3–5 mM range. They were sourced from water and sludge samples obtained from sewage treatment facilities in a large city. Outcomes of phylogenetic analysis of 16S rRNA gene sequences revealed that tolerant strains of bacteria belonged to the genera Pseudomonas , Serratia and Klebsiella ; strains belonging to the genus Pseudomonas dominated. Of ten resistant strains, nine were isolated from sludge and water samples of the secondary sedimentation tank, and the other one from a treatment plant’s digester. Changes in the color of microorganisms’ colonies became evident when cultivated on media enriched with heavy metals. Cultivating nonpathogenic strains of these bacteria and their introduction into communities of other activated sludge microorganisms could have practical application to biological decontamination of wastewater.

Suggested Citation

  • Leonid Perelomov & Olga Sizova & Mohammad Mahmudur Rahman & Irina Perelomova & Tatiana Minkina & Sergei Sokolov & Yury Atroshchenko, 2022. "Metal-Tolerant Bacteria of Wastewater Treatment Plant in a Large City," Sustainability, MDPI, vol. 14(18), pages 1-11, September.
    • Handle: RePEc:gam:jsusta:v:14:y:2022:i:18:p:11335-:d:911243
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    References listed on IDEAS

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    1. Abdul Wahab Ajmal & Saleha Saroosh & Shah Mulk & Muhammad Nadeem Hassan & Humaira Yasmin & Zahra Jabeen & Asia Nosheen & Syed Muhammad Usman Shah & Rabia Naz & Zuhair Hasnain & Tariq Mahmood Qureshi &, 2021. "Bacteria Isolated from Wastewater Irrigated Agricultural Soils Adapt to Heavy Metal Toxicity While Maintaining Their Plant Growth Promoting Traits," Sustainability, MDPI, vol. 13(14), pages 1-15, July.
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