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Exploring Functional Diversity and Community Structure of Diazotrophic Endophytic Bacteria Associated with Pennisetum glaucum Growing under Field in a Semi-Arid Region

Author

Listed:
  • Garima Gupta

    (Institute of Bioscience and Technology, Shri Ramswaroop Memorial University, Lucknow-Deva Road, Barabanki 225003, India)

  • Sangeeta Paul

    (Indian Agricultural Research Institute, Pusa, New Delhi 110012, India)

  • Sachidanand Singh

    (Department of Biotechnology, Vignan’s Foundation for Science, Technology and Research, Vadlamudi, Guntur 522213, India
    Department of Biotechnology, Smt. S. S. Patel Nootan Science & Commerce College, Sankalchand Patel University, Visnagar 384315, India)

  • Giacomo Pietramellara

    (Department of Agriculture, Food, Environment and Forestry, University of Florence, P.le delle Cascine 28, 50144 Firenze, Italy)

  • Shamina Imran Pathan

    (Department of Agriculture, Food, Environment and Forestry, University of Florence, P.le delle Cascine 28, 50144 Firenze, Italy)

  • Subhan Danish

    (Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresource, College of Tropical Crops, Hainan University, Haikou 570228, China)

  • Dilfuza Jabborova

    (Institute of Genetics and Plant Experimental Biology, Uzbekistan Academy of Sciences, Kibray 111208, Uzbekistan)

  • Rahul Datta

    (Department of Geology and Pedology, Faculty of Forestry and Wood Technology, Mendel University in Brno, Zemedelska 1, 61300 Brno, Czech Republic)

  • Prabhat Nath Jha

    (Centre for Biotechnology, Department of Biological Sciences, Birla Institute of Technology and Science, Pilani 333031, India)

Abstract

Diazotrophic endophytic bacteria (DEB) are the key drivers of nitrogen fixation in rainfed soil ecosystems and, hence, can influence the growth and yield of crop plants. Therefore, the present work investigated the structure and composition of the DEB community at different growth stages of field-grown pearl millet plants, employing the cultivation-dependent method. Diazotrophy of the bacterial isolates was confirmed by acetylene reduction assay and amplification of the nifH gene. ERIC-PCR-based DNA fingerprinting, followed by 16S rRNA gene analysis of isolates recovered at different time intervals, demonstrated the highest bacterial diversity during early (up to 28 DAS (Days after sowing)) and late (63 DAS onwards) stages, as compared to the vegetative growth stage (28–56 DAS). Among all species, Pseudomonas aeruginosa was the most dominant endophyte. Assuming modulation of the immune response as one of the tactics for successful colonization of P. aeruginosa PM389, we studied the expression of the profile of defense genes of wheat, used as a host plant, in response to P. aeruginosa inoculation. Most of the pathogenesis-related PR genes were induced initially (at 6 h after infection (HAI)), followed by their downregulation at 12 HAI. The trend of bacterial colonization was quantified by qPCR of 16S rRNAs. The results obtained in the present study indicated an attenuated defense response in host plants towards endophytic bacteria, which is an important feature that helps endophytes establish themselves inside the endosphere of roots.

Suggested Citation

  • Garima Gupta & Sangeeta Paul & Sachidanand Singh & Giacomo Pietramellara & Shamina Imran Pathan & Subhan Danish & Dilfuza Jabborova & Rahul Datta & Prabhat Nath Jha, 2022. "Exploring Functional Diversity and Community Structure of Diazotrophic Endophytic Bacteria Associated with Pennisetum glaucum Growing under Field in a Semi-Arid Region," Land, MDPI, vol. 11(7), pages 1-24, June.
    • Handle: RePEc:gam:jlands:v:11:y:2022:i:7:p:991-:d:851566
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    References listed on IDEAS

    as
    1. Muhammad Zafar-ul-Hye & Muhammad Naeem Akbar & Yasir Iftikhar & Mazhar Abbas & Atiqa Zahid & Shah Fahad & Rahul Datta & Muqarrab Ali & Abdallah M. Elgorban & Mohammad Javed Ansari & Subhan Danish, 2021. "Rhizobacteria Inoculation and Caffeic Acid Alleviated Drought Stress in Lentil Plants," Sustainability, MDPI, vol. 13(17), pages 1-14, August.
    2. Deepranjan Sarkar & Amitava Rakshit & Ahmad I. Al-Turki & R. Z. Sayyed & Rahul Datta, 2021. "Connecting Bio-Priming Approach with Integrated Nutrient Management for Improved Nutrient Use Efficiency in Crop Species," Agriculture, MDPI, vol. 11(4), pages 1-18, April.
    3. Deepranjan Sarkar & Amitava Rakshit & Hanuman Prasad Parewa & Subhan Danish & Saleh Alfarraj & Rahul Datta, 2022. "Bio-Priming with Compatible Rhizospheric Microbes Enhances Growth and Micronutrient Uptake of Red Cabbage," Land, MDPI, vol. 11(4), pages 1-20, April.
    4. Rahul Datta & Divyashri Baraniya & Yong-Feng Wang & Aditi Kelkar & Ram Swaroop Meena & Gulab Singh Yadav & Maria Teresa Ceccherini & Pavel Formanek, 2017. "Amino Acid: Its Dual Role as Nutrient and Scavenger of Free Radicals in Soil," Sustainability, MDPI, vol. 9(8), pages 1-9, August.
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