IDEAS home Printed from https://ideas.repec.org/a/gam/jijerp/v19y2022i5p3108-d765331.html
   My bibliography  Save this article

Optimization of Lipid Production by Schizochytrium limacinum Biomass Modified with Ethyl Methane Sulfonate and Grown on Waste Glycerol

Author

Listed:
  • Szymon Talbierz

    (InnovaTree Sp. z o.o., 81-451 Gdynia, Poland)

  • Marcin Dębowski

    (Department of Environment Engineering, Faculty of Geoengineering, University of Warmia and Mazury in Olsztyn, 10-720 Olsztyn, Poland)

  • Natalia Kujawska

    (InnovaTree Sp. z o.o., 81-451 Gdynia, Poland)

  • Joanna Kazimierowicz

    (Department of Water Supply and Sewage Systems, Faculty of Civil Engineering and Environmental Sciences, Bialystok University of Technology, 15-351 Bialystok, Poland)

  • Marcin Zieliński

    (Department of Environment Engineering, Faculty of Geoengineering, University of Warmia and Mazury in Olsztyn, 10-720 Olsztyn, Poland)

Abstract

One of the most promising avenues of biofuel research relates to using waste as a starting feedstock to produce liquid or gaseous energy carriers. The global production of waste glycerol by the refinery industry is rising year after year. The aim of the present study was to examine the effect of ethyl methane sulfonate (EMS) on the growth rates and intracellular lipid accumulation in heterotrophically-cultured Schizochytrium limacinum microalgae, grown on waste glycerol as the carbon source. The strain S. limacinum E20, produced by incubating a reference strain in EMS for 20 min, was found to perform the best in terms of producing biomass (0.054 g DW /dm 3 ·h) and accumulating intracellular bio-oil (0.021 g/dm 3 ·h). The selected parameters proved to be optimal for S. limacinum E20 biomass growth at the following values: temperature 27.3 °C, glycerol level 249.0 g/dm 3 , oxygen in the culture 26%, and yeast extract concentration 45.0 g/dm 3 . In turn, the optimal values for lipid production in an S. limacinum E20 culture were: temperature 24.2 °C, glycerol level 223.0 g/dm 3 , oxygen in the culture 10%, and yeast extract concentration 10.0 g/dm 3 . As the process conditions are different for biomass growth and for intracellular lipid accumulation, it is recommended to use a two-step culture process, which resulted in a lipid synthesis rate of 0.41 g/dm 3 ·h.

Suggested Citation

  • Szymon Talbierz & Marcin Dębowski & Natalia Kujawska & Joanna Kazimierowicz & Marcin Zieliński, 2022. "Optimization of Lipid Production by Schizochytrium limacinum Biomass Modified with Ethyl Methane Sulfonate and Grown on Waste Glycerol," IJERPH, MDPI, vol. 19(5), pages 1-17, March.
    • Handle: RePEc:gam:jijerp:v:19:y:2022:i:5:p:3108-:d:765331
    as

    Download full text from publisher

    File URL: https://www.mdpi.com/1660-4601/19/5/3108/pdf
    Download Restriction: no
    File URL: https://www.mdpi.com/1660-4601/19/5/3108/
    Download Restriction: no
    ---><---

    References listed on IDEAS

    as
    1. Natalia Kujawska & Szymon Talbierz & Marcin Dębowski & Joanna Kazimierowicz & Marcin Zieliński, 2021. "Cultivation Method Effect on Schizochytrium sp. Biomass Growth and Docosahexaenoic Acid (DHA) Production with the Use of Waste Glycerol as a Source of Organic Carbon," Energies, MDPI, vol. 14(10), pages 1-16, May.
    2. Mihaela Simionescu & Yuriy Bilan & Piotr Zawadzki & Adam Wojciechowski & Marcin Rabe, 2021. "GHG Emissions Mitigation in the European Union Based on Labor Market Changes," Energies, MDPI, vol. 14(2), pages 1-13, January.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Marcin Zieliński & Marcin Dębowski & Joanna Kazimierowicz, 2022. "Outflow from a Biogas Plant as a Medium for Microalgae Biomass Cultivation—Pilot Scale Study and Technical Concept of a Large-Scale Installation," Energies, MDPI, vol. 15(8), pages 1-18, April.
    2. Anna Misztal & Magdalena Kowalska & Anita Fajczak-Kowalska & Otakar Strunecky, 2021. "Energy Efficiency and Decarbonization in the Context of Macroeconomic Stabilization," Energies, MDPI, vol. 14(16), pages 1-18, August.
    3. Marcin Zieliński & Marcin Dębowski & Joanna Kazimierowicz & Izabela Świca, 2023. "Microalgal Carbon Dioxide (CO 2 ) Capture and Utilization from the European Union Perspective," Energies, MDPI, vol. 16(3), pages 1-27, February.
    4. Olga Pilipczuk, 2021. "Determinants of Managerial Competences Transformation in the Polish Energy Industry," Energies, MDPI, vol. 14(20), pages 1-27, October.
    5. Marcin Dębowski & Izabela Świca & Joanna Kazimierowicz & Marcin Zieliński, 2022. "Large Scale Microalgae Biofuel Technology—Development Perspectives in Light of the Barriers and Limitations," Energies, MDPI, vol. 16(1), pages 1-23, December.
    6. Anna Misztal & Magdalena Kowalska & Anita Fajczak-Kowalska, 2022. "The Impact of Economic Factors on the Sustainable Development of Energy Enterprises: The Case of Bulgaria, Czechia, Estonia and Poland," Energies, MDPI, vol. 15(18), pages 1-19, September.
    7. Mihaela Simionescu & Adam Wojciechowski & Arkadiusz Tomczyk & Marcin Rabe, 2021. "Revised Environmental Kuznets Curve for V4 Countries and Baltic States," Energies, MDPI, vol. 14(11), pages 1-15, June.
    8. Mihaela Simionescu & Carmen Beatrice Păuna & Mihaela-Daniela Vornicescu Niculescu, 2021. "The Relationship between Economic Growth and Pollution in Some New European Union Member States: A Dynamic Panel ARDL Approach," Energies, MDPI, vol. 14(9), pages 1-17, April.
    9. Wojciech Drożdż & Grzegorz Kinelski & Marzena Czarnecka & Magdalena Wójcik-Jurkiewicz & Anna Maroušková & Grzegorz Zych, 2021. "Determinants of Decarbonization—How to Realize Sustainable and Low Carbon Cities?," Energies, MDPI, vol. 14(9), pages 1-19, May.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:gam:jijerp:v:19:y:2022:i:5:p:3108-:d:765331. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: MDPI Indexing Manager (email available below). General contact details of provider: https://www.mdpi.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.