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First Report of an Asymptomatic Leishmania (Viannia) shawi Infection Using a Nasal Swab in Amazon, Brazil

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  • Luciana P. Oliveira

    (Ministério da Saúde, Secretaria de Vigilância em Saúde, Instituto Evandro Chagas, Ananindeua 67015-120, PA, Brazil)

  • Luciana C. S. Nascimento

    (Ministério da Saúde, Secretaria de Vigilância em Saúde, Instituto Evandro Chagas, Ananindeua 67015-120, PA, Brazil)

  • Fabiola S. Santos

    (Ministério da Saúde, Secretaria de Vigilância em Saúde, Instituto Evandro Chagas, Ananindeua 67015-120, PA, Brazil)

  • Jaqueline L. C. Takamatsu

    (Hospital Municipal de Tomé Açu, Tomé-Açu 68680-000, PA, Brazil)

  • Luiz R. P. Sanchez

    (Hospital Municipal de Tomé Açu, Tomé-Açu 68680-000, PA, Brazil)

  • Walter S. Santos

    (Ministério da Saúde, Secretaria de Vigilância em Saúde, Instituto Evandro Chagas, Ananindeua 67015-120, PA, Brazil)

  • Lourdes M. Garcez

    (Ministério da Saúde, Secretaria de Vigilância em Saúde, Instituto Evandro Chagas, Ananindeua 67015-120, PA, Brazil
    Departamento de Patologia, Universidade do Estado do Pará, Belém 66050-540, PA, Brazil)

Abstract

The state of Pará has recorded seven Leishmania species that cause tegumentary leishmaniasis (TL). Leishmania species induce distinct immunological responses from the host and exhibit resistance to Glucantime, the first-line drug treatment for TL in Brazil. Objective: Identify the etiology of TL in an Amazonian city in the state of Pará. Material and methods: Eleven patients with TL were recruited and nasal swabs, lesion swabs, and skin fragments samples were collected. In the control group (n = 6), only the nasal swabs were collected. Polymerase Chain Reaction (PCR) amplification of the gene region hsp70-234 was performed using the extracted DNA from the samples, from which nine patients with TL and five in the control group were positive. Products were sequenced, mounted in CAP3 software, aligned using MAFFT v.7.221, edited in Geneious software v.8.1.7, and compared and aligned with sequences available in GenBank using the BLAST tool. Results: For patients with TL, six molecular diagnosis at the species level ( L. ( Viannia ) braziliensis (n = 5/9), L. ( Viannia ) shawi (n = 1/9)) and three at the genus level ( Leishmania sp. (n = 3/9)) were obtained. In the control group, four individuals were infected with Leishmania sp. (n = 4/5) and L. (V.) shawi (n = 1/5). Conclusion: This is the first report of L. (V.) shawi infection in the mucosal secretion of a healthy person in Brazil. Moreover, genetic variants were identified in the haplotypes of L. (V.) braziliensis in the gene sequence hsp70-234.

Suggested Citation

  • Luciana P. Oliveira & Luciana C. S. Nascimento & Fabiola S. Santos & Jaqueline L. C. Takamatsu & Luiz R. P. Sanchez & Walter S. Santos & Lourdes M. Garcez, 2022. "First Report of an Asymptomatic Leishmania (Viannia) shawi Infection Using a Nasal Swab in Amazon, Brazil," IJERPH, MDPI, vol. 19(10), pages 1-10, May.
    • Handle: RePEc:gam:jijerp:v:19:y:2022:i:10:p:6346-:d:822149
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    References listed on IDEAS

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    1. Luz Estella Mesa & Rubén Manrique & Carlos Muskus & Sara M Robledo, 2020. "Test accuracy of polymerase chain reaction methods against conventional diagnostic techniques for Cutaneous Leishmaniasis (CL) in patients with clinical or epidemiological suspicion of CL: Systematic ," PLOS Neglected Tropical Diseases, Public Library of Science, vol. 14(1), pages 1-15, January.
    2. Francisco J. A. Santos & Luciana C. S. Nascimento & Wellington B. Silva & Luciana P. Oliveira & Walter S. Santos & Délia C. F. Aguiar & Lourdes M. Garcez, 2020. "First Report of Canine Infection by Leishmania ( Viannia ) guyanensis in the Brazilian Amazon," IJERPH, MDPI, vol. 17(22), pages 1-9, November.
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