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Occupational Exposure in Industrial Painters: Sensitive and Noninvasive Biomarkers to Evaluate Early Cytotoxicity, Genotoxicity and Oxidative Stress

Author

Listed:
  • Delia Cavallo

    (Department of Occupational and Environmental Medicine, Epidemiology and Hygiene, Italian Workers’ Compensation Authority—INAIL, Monte Porzio Catone, 00078 Rome, Italy)

  • Cinzia Lucia Ursini

    (Department of Occupational and Environmental Medicine, Epidemiology and Hygiene, Italian Workers’ Compensation Authority—INAIL, Monte Porzio Catone, 00078 Rome, Italy)

  • Anna Maria Fresegna

    (Department of Occupational and Environmental Medicine, Epidemiology and Hygiene, Italian Workers’ Compensation Authority—INAIL, Monte Porzio Catone, 00078 Rome, Italy)

  • Aureliano Ciervo

    (Department of Occupational and Environmental Medicine, Epidemiology and Hygiene, Italian Workers’ Compensation Authority—INAIL, Monte Porzio Catone, 00078 Rome, Italy)

  • Raffaele Maiello

    (Department of Occupational and Environmental Medicine, Epidemiology and Hygiene, Italian Workers’ Compensation Authority—INAIL, Monte Porzio Catone, 00078 Rome, Italy)

  • Giuliana Buresti

    (Department of Occupational and Environmental Medicine, Epidemiology and Hygiene, Italian Workers’ Compensation Authority—INAIL, Monte Porzio Catone, 00078 Rome, Italy)

  • Enrico Paci

    (Department of Occupational and Environmental Medicine, Epidemiology and Hygiene, Italian Workers’ Compensation Authority—INAIL, Monte Porzio Catone, 00078 Rome, Italy)

  • Daniela Pigini

    (Department of Occupational and Environmental Medicine, Epidemiology and Hygiene, Italian Workers’ Compensation Authority—INAIL, Monte Porzio Catone, 00078 Rome, Italy)

  • Monica Gherardi

    (Department of Occupational and Environmental Medicine, Epidemiology and Hygiene, Italian Workers’ Compensation Authority—INAIL, Monte Porzio Catone, 00078 Rome, Italy)

  • Damiano Carbonari

    (Department of Occupational and Environmental Medicine, Epidemiology and Hygiene, Italian Workers’ Compensation Authority—INAIL, Monte Porzio Catone, 00078 Rome, Italy)

  • Renata Sisto

    (Department of Occupational and Environmental Medicine, Epidemiology and Hygiene, Italian Workers’ Compensation Authority—INAIL, Monte Porzio Catone, 00078 Rome, Italy)

  • Giovanna Tranfo

    (Department of Occupational and Environmental Medicine, Epidemiology and Hygiene, Italian Workers’ Compensation Authority—INAIL, Monte Porzio Catone, 00078 Rome, Italy)

  • Sergio Iavicoli

    (Department of Occupational and Environmental Medicine, Epidemiology and Hygiene, Italian Workers’ Compensation Authority—INAIL, Monte Porzio Catone, 00078 Rome, Italy)

Abstract

This study aimed to identify sensitive and noninvasive biomarkers of early cyto-genotoxic, oxidative and inflammatory effects for exposure to volatile organic compounds (VOCs) in shipyard painters. On 17 (11 spray and 6 roller) painters (previously characterized for VOCs exposure to toluene, xylenes, ethylbenzene, ethyl acetate) and on 18 controls, we performed buccal micronucleus cytome (BMCyt) assay; Fpg-comet assay on lymphocytes; detection of urinary 8-oxoGua (8-oxo-7,8-dihydroguanine), 8-oxodGuo (8-oxo-7,8-dihydro-2′-deoxyguanosine) and 8-oxoGuo (8-oxo-7,8-dihydroguanosine), and cytokines release on serum. We found induction of cyto-genotoxicity by BMCyt assay and inflammatory effects (IL-6 and TNFα) in roller painters exposed to lower VOC concentrations than spray painters. In contrast, in both worker groups, we found direct and oxidative DNA damage by comet assay (with slightly higher oxidative DNA damage in roller) and significant increase of 8-oxoGuo and decrease of 8-oxodGuo and 8-oxoGua in respect to controls. The cyto-genotoxicity observed only on buccal cells of roller painters could be related to the task’s specificity and the different used protective equipment. Although limited by the small number of subjects, the study shows the usefulness of all the used biomarkers in the risk assessment of painters workers exposed to complex mixtures.

Suggested Citation

  • Delia Cavallo & Cinzia Lucia Ursini & Anna Maria Fresegna & Aureliano Ciervo & Raffaele Maiello & Giuliana Buresti & Enrico Paci & Daniela Pigini & Monica Gherardi & Damiano Carbonari & Renata Sisto &, 2021. "Occupational Exposure in Industrial Painters: Sensitive and Noninvasive Biomarkers to Evaluate Early Cytotoxicity, Genotoxicity and Oxidative Stress," IJERPH, MDPI, vol. 18(9), pages 1-14, April.
    • Handle: RePEc:gam:jijerp:v:18:y:2021:i:9:p:4645-:d:544472
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    References listed on IDEAS

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    1. Giovanna Tranfo & Enrico Paci & Mariella Carrieri & Enrico Marchetti & Renata Sisto & Monica Gherardi & Francesca Costabile & Lisa Bauleo & Carla Ancona & Daniela Pigini, 2019. "Levels of Urinary Biomarkers of Oxidatively Generated Damage to DNA and RNA in Different Groups of Workers Compared to General Population," IJERPH, MDPI, vol. 16(16), pages 1-13, August.
    2. Giovanna Tranfo, 2020. "The Growing Importance of the Human Biomonitoring of Exposure," IJERPH, MDPI, vol. 17(11), pages 1-3, June.
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