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Effects of 220 MHz Pulsed Modulated Radiofrequency Field on the Sperm Quality in Rats

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  • Ling Guo

    (Department of Radiation Biology, Faculty of Preventive Medicine, Fourth Military Medical University, 169# Chang Le West Road, Xi’an 710032, China
    Ministry of Education Key Lab of Hazard Assessment and Control in Special Operational Environment, 169# Chang Le West Road, Xi’an 710032, China
    These authors contributed equally to the work.)

  • Jia-Jin Lin

    (Department of Radiation Biology, Faculty of Preventive Medicine, Fourth Military Medical University, 169# Chang Le West Road, Xi’an 710032, China
    Ministry of Education Key Lab of Hazard Assessment and Control in Special Operational Environment, 169# Chang Le West Road, Xi’an 710032, China
    These authors contributed equally to the work.)

  • Yi-Zhe Xue

    (Department of Radiation Biology, Faculty of Preventive Medicine, Fourth Military Medical University, 169# Chang Le West Road, Xi’an 710032, China)

  • Guang-Zhou An

    (Department of Radiation Biology, Faculty of Preventive Medicine, Fourth Military Medical University, 169# Chang Le West Road, Xi’an 710032, China
    Ministry of Education Key Lab of Hazard Assessment and Control in Special Operational Environment, 169# Chang Le West Road, Xi’an 710032, China)

  • Jun-Ping Zhang

    (Department of Radiation Biology, Faculty of Preventive Medicine, Fourth Military Medical University, 169# Chang Le West Road, Xi’an 710032, China)

  • Ke-Ying Zhang

    (Department of Radiation Biology, Faculty of Preventive Medicine, Fourth Military Medical University, 169# Chang Le West Road, Xi’an 710032, China)

  • Wei He

    (Department of Radiation Biology, Faculty of Preventive Medicine, Fourth Military Medical University, 169# Chang Le West Road, Xi’an 710032, China
    Ministry of Education Key Lab of Hazard Assessment and Control in Special Operational Environment, 169# Chang Le West Road, Xi’an 710032, China)

  • Huan Wang

    (Department of Radiation Biology, Faculty of Preventive Medicine, Fourth Military Medical University, 169# Chang Le West Road, Xi’an 710032, China
    Ministry of Education Key Lab of Hazard Assessment and Control in Special Operational Environment, 169# Chang Le West Road, Xi’an 710032, China)

  • Wei Li

    (Department of Histology and Embryology, Fourth Military Medical University, 169# Chang Le West Road, Xi’an 710032, China)

  • Gui-Rong Ding

    (Department of Radiation Biology, Faculty of Preventive Medicine, Fourth Military Medical University, 169# Chang Le West Road, Xi’an 710032, China
    Ministry of Education Key Lab of Hazard Assessment and Control in Special Operational Environment, 169# Chang Le West Road, Xi’an 710032, China)

Abstract

Under some occupational conditions, workers are inevitably exposed to high-intensity radiofrequency (RF) fields. In this study, we investigated the effects of one-month exposure to a 220 MHz pulsed modulated RF field at the power density of 50 W/m 2 on the sperm quality in male adult rats. The sperm quality was evaluated by measuring the number, abnormality and survival rate of sperm cells. The morphology of testis was examined by hematoxylin–eosin (HE) staining. The levels of secreting factors by Sertoli cells (SCs) and Leydig cells (LCs) were determined by enzyme linked immunosorbent assay (ELISA). The level of cleaved caspase 3 in the testis was detected by immunofluorescence staining. Finally, the expression levels of the apoptosis-related protein (caspase 3, BAX and BCL2) in the testis were assessed by Western blotting. Compared with the sham group, the sperm quality in the RF group decreased significantly. The levels of secreting factors of SCs and the morphology of the testis showed an obvious change after RF exposure. The level of the secreting factor of LCs decreased significantly after RF exposure. The levels of cleaved caspase 3, caspase 3, and the BAX/BCL2 ratio in the testis increased markedly after RF exposure. These data collectively suggested that under the present experimental conditions, 220 MHz pulsed modulated RF exposure could impair sperm quality in rats, and the disruption of the secreting function of LCs and increased apoptosis of testis cells induced by the RF field might be accounted for by this damaging effect.

Suggested Citation

  • Ling Guo & Jia-Jin Lin & Yi-Zhe Xue & Guang-Zhou An & Jun-Ping Zhang & Ke-Ying Zhang & Wei He & Huan Wang & Wei Li & Gui-Rong Ding, 2019. "Effects of 220 MHz Pulsed Modulated Radiofrequency Field on the Sperm Quality in Rats," IJERPH, MDPI, vol. 16(7), pages 1-12, April.
    • Handle: RePEc:gam:jijerp:v:16:y:2019:i:7:p:1286-:d:221584
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    References listed on IDEAS

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    1. Tan Ma & Xiaoqin Yin & Ruitong Han & Jie Ding & Huan Zhang & Xiaodong Han & Dongmei Li, 2017. "Effects of In Utero Exposure to Di-n-Butyl Phthalate on Testicular Development in Rat," IJERPH, MDPI, vol. 14(10), pages 1-12, October.
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    Cited by:

    1. Andrea Vornoli & Laura Falcioni & Daniele Mandrioli & Luciano Bua & Fiorella Belpoggi, 2019. "The Contribution of In Vivo Mammalian Studies to the Knowledge of Adverse Effects of Radiofrequency Radiation on Human Health," IJERPH, MDPI, vol. 16(18), pages 1-27, September.
    2. Maria Rosaria Scarfì & Mats-Olof Mattsson & Myrtill Simkó & Olga Zeni, 2019. "Special Issue: “Electric, Magnetic, and Electromagnetic Fields in Biology and Medicine: From Mechanisms to Biomedical Applications”," IJERPH, MDPI, vol. 16(22), pages 1-3, November.

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