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Study of the In Vitro Antagonistic Activity of Various Single-Strain and Multi-Strain Probiotics against Escherichia coli

Author

Listed:
  • Sabina Fijan

    (Faculty of Health Sciences, University of Maribor, Žitna ulica 15, 2000 Maribor, Slovenia)

  • Dunja Šulc

    (Faculty of Health Sciences, University of Maribor, Žitna ulica 15, 2000 Maribor, Slovenia)

  • Andrej Steyer

    (Faculty of Medicine, Institute of Microbiology and Immunology, University of Ljubljana, Zaloška 4, 1000 Ljubljana, Slovenia)

Abstract

Escherichia coli is an important commensal of our gut, however, many pathogenic strains exist, causing various severe infections in the gut or beyond. Due to several antibiotic resistance patterns of E. coli , research of alternative treatments or adjuvant therapy is important. One of these is the use of probiotics as antagonistic agents against E. coli . Most published studies investigate only one strain of E. coli and single-strain probiotics. The objectives of this study were to evaluate the antagonistic activity of selected single-strain and multi-strain probiotic supplements against selected clinical E. coli pathotypes using the in vitro agar spot test and the co-culturing method. Molecular methods were used to determine the presence of the genus lactobacilli and bifidobacteria as well as certain selected strains in the probiotic supplements. The agar-spot test showed that the multi-strain probiotics were more effective than the single-strain probiotics. On the other hand, the co-culturing method showed the opposite result, indicating that results are importantly influenced by the chosen method. The most effective single-strain probiotics against E. coli strains were Bifidobacterium animalis subsp. lactis BB-12 and Lactobacillus reuteri DSM 17938. The most effective multi-strain probiotics contained lactobacilli, bifidobacteria and enterococci strains, thus proving that most effective probiotics against E. coli strains are the lactic acid bacteria and bifidobacteria. The overall results from both in vitro tests reveal that all selected probiotics exhibited an antagonistic activity against all E. coli strains. From a public health perspective probiotics have thus proved to be successful in inhibiting the growth of E. coli and could therefore be used as adjuvant therapy or alternative therapy in E. coli infections.

Suggested Citation

  • Sabina Fijan & Dunja Šulc & Andrej Steyer, 2018. "Study of the In Vitro Antagonistic Activity of Various Single-Strain and Multi-Strain Probiotics against Escherichia coli," IJERPH, MDPI, vol. 15(7), pages 1-15, July.
    • Handle: RePEc:gam:jijerp:v:15:y:2018:i:7:p:1539-:d:159009
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    References listed on IDEAS

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    1. Raphael S. Steinberg & Lilian C. S. Silva & Tássia C. Souza & Maurício T. Lima & Nayara L. G. De Oliveira & Leda Q. Vieira & Rosa M. E. Arantes & Anderson Miyoshi & Jacques R. Nicoli & Elisabeth Neuma, 2014. "Safety and Protective Effectiveness of Two Strains of Lactobacillus with Probiotic Features in an Experimental Model of Salmonellosis," IJERPH, MDPI, vol. 11(9), pages 1-22, August.
    2. Mokaba Shirley Malema & Akebe Luther King Abia & Roman Tandlich & Bonga Zuma & Jean-Marc Mwenge Kahinda & Eunice Ubomba-Jaswa, 2018. "Antibiotic-Resistant Pathogenic Escherichia Coli Isolated from Rooftop Rainwater-Harvesting Tanks in the Eastern Cape, South Africa," IJERPH, MDPI, vol. 15(5), pages 1-14, May.
    3. Sabina Fijan, 2014. "Microorganisms with Claimed Probiotic Properties: An Overview of Recent Literature," IJERPH, MDPI, vol. 11(5), pages 1-23, May.
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    1. Sabina Fijan & Anita Frauwallner & László Varga & Tomaž Langerholc & Irena Rogelj & Mateja Lorber & Peter Lewis & Petra Povalej Bržan, 2019. "Health Professionals’ Knowledge of Probiotics: An International Survey," IJERPH, MDPI, vol. 16(17), pages 1-16, August.

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