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Inoculant Formulation for Bradyrhizobium spp.: Optimizing CMC/Starch Blends for Improved Performance

Author

Listed:
  • Jaqueline Carvalho de Almeida

    (Biological Resource Center, CRB, Embrapa Agrobiology, Seropedica 23897-970, RJ, Brazil
    Current address: Postgraduate Program in Crop Science, Federal Rural University of Rio de Janeiro, Seropedica 23897-000, RJ, Brazil.)

  • Vinício Oliosi Favero

    (Capixaba Institute for Research, Technical Assistance and Rural Extension, INCAPER, Saint Gabriel of Straw 29780-000, ES, Brazil
    Current address: Postgraduate Program in Soil Science, Federal Rural University of Rio de Janeiro, Seropedica 23897-000, RJ, Brazil.)

  • Janaina Ribeiro Costa Rouws

    (Embrapa Agrobiology, Seropedica 23897-970, RJ, Brazil)

  • Carla de Sant’Anna Freitas

    (OCP Brasil, Sao Paulo 04543-011, SP, Brazil
    Current address: Postgraduate Program in Chemical Engineering, Federal Rural University of Rio de Janeiro, Seropedica 23897-000, RJ, Brazil.)

  • Érica Barbosa de Sousa

    (Institute of Chemistry, Federal Rural University of Rio de Janeiro, UFRRJ, Seropedica 23897-000, RJ, Brazil
    Current address: Postgraduate Program in Chemical Engineering, Federal Rural University of Rio de Janeiro, Seropedica 23897-000, RJ, Brazil.)

  • Jéssica Feitoza da Rocha

    (Abbott Laboratories, Rio de Janeiro 22710-907, RJ, Brazil
    Current address: Postgraduate Program in Chemical Engineering, Federal Rural University of Rio de Janeiro, Seropedica 23897-000, RJ, Brazil.)

  • Nainicelle Cibelle Sousa Chantre

    (Ministry of Education of Cape Verde, Beach, Praia 7600, Cape Verde
    Current address: Postgraduate Program in Chemical Engineering, Federal Rural University of Rio de Janeiro, Seropedica 23897-000, RJ, Brazil.)

  • Gustavo Ribeiro Xavier

    (Embrapa Agrobiology, Seropedica 23897-970, RJ, Brazil)

  • Paulo Jansen de Oliveira

    (Department of Chemical Engineering, Federal Rural University of Rio de Janeiro, UFRRJ, Seropedica 23897-000, RJ, Brazil)

  • Norma Gouvêa Rumjanek

    (Embrapa Agrobiology, Seropedica 23897-970, RJ, Brazil)

Abstract

Inoculating legumes with nitrogen-fixing bacteria, such as Bradyrhizobium , can significantly reduce reliance on synthetic nitrogen fertilizers. To optimize this process, a suitable rhizobial strain must be carefully selected and formulated. This study aimed to develop a biopolymer blend formulation for Bradyrhizobium pachyrhizi strain BR 3262. From four commercial starches and two carboxymethylcelluloses (CMC), we developed CMC/starch blends compatibilized or not with MgO at concentrations from 0.1% to 1.0% and subjected them to autoclaving for either 30 or 60 min. The resulting inoculants were stored for 168 days. Generally, blends compatibilized with 1.0% MgO exhibited a significant decrease in cell numbers, likely due to the observed pH values of approximately 10. The best performance was observed for CMC-I/starch B blends autoclaved for 60 min, and CMC-II/starch C blends autoclaved for 30 min, both compatibilized with 0.3% MgO. These blends maintained a cell viability of 10 8 CFU mL −1 for approximately 130 days at room temperature. Blend optimization depends on the selection of specific interactions and quantities of each component in order to achieve a given functionality; in the conditions of this study, the capacity to maintain Bradyrhizobium cell viability for at least four months.

Suggested Citation

  • Jaqueline Carvalho de Almeida & Vinício Oliosi Favero & Janaina Ribeiro Costa Rouws & Carla de Sant’Anna Freitas & Érica Barbosa de Sousa & Jéssica Feitoza da Rocha & Nainicelle Cibelle Sousa Chantre , 2025. "Inoculant Formulation for Bradyrhizobium spp.: Optimizing CMC/Starch Blends for Improved Performance," Agriculture, MDPI, vol. 15(9), pages 1-16, May.
    • Handle: RePEc:gam:jagris:v:15:y:2025:i:9:p:1010-:d:1650614
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