IDEAS home Printed from https://ideas.repec.org/a/gam/jagris/v15y2025i15p1591-d1709171.html
   My bibliography  Save this article

Baseline Sensitivity of Leptosphaeria maculans to Succinate Dehydrogenase Inhibitor (SDHI) Fungicides and Development of Molecular Markers for Future Monitoring

Author

Listed:
  • Alec J. McCallum

    (School of BioSciences, University of Melbourne, Victoria 3010, Australia)

  • Alexander Idnurm

    (School of BioSciences, University of Melbourne, Victoria 3010, Australia)

  • Angela P. Van de Wouw

    (School of BioSciences, University of Melbourne, Victoria 3010, Australia)

Abstract

Succinate dehydrogenase inhibitor (SDHI) fungicides are widely used in Australia for the control of blackleg disease (caused by Leptosphaeria maculans , also called Plenodomus lingam ). Populations of L. maculans are highly variable and therefore at risk of evolving fungicide resistance. The baseline sensitivities of L. maculans isolates towards the SDHI fungicides pydiflumetofen and bixafen were determined through in vitro mycelial growth assays, and the mean EC 50 s were found to be 4.89 and 2.71 ng mL −1 , respectively. L. maculans populations were also screened against three commercial SDHI fungicides, Saltro ® , ILeVO ® , and Aviator ® , using an in planta assay to reveal very low levels of resistance. Nineteen of these ascospore populations from 2022 were analysed in a deep amplicon sequencing (DAS) assay and showed no mutations in the genes likely to be associated with resistance to SDHI chemistries. This study establishes baseline sensitivities of L. maculans isolates towards commonly used SDHI fungicides, importantly before and during the introduction of these new chemistries for blackleg control, and outlines monitoring techniques to allow timely identification of resistance if it evolves.

Suggested Citation

  • Alec J. McCallum & Alexander Idnurm & Angela P. Van de Wouw, 2025. "Baseline Sensitivity of Leptosphaeria maculans to Succinate Dehydrogenase Inhibitor (SDHI) Fungicides and Development of Molecular Markers for Future Monitoring," Agriculture, MDPI, vol. 15(15), pages 1-14, July.
    • Handle: RePEc:gam:jagris:v:15:y:2025:i:15:p:1591-:d:1709171
    as

    Download full text from publisher

    File URL: https://www.mdpi.com/2077-0472/15/15/1591/pdf
    Download Restriction: no
    File URL: https://www.mdpi.com/2077-0472/15/15/1591/
    Download Restriction: no
    ---><---

    References listed on IDEAS

    as
    1. Olufadekemi Fajemisin & Jana Mazáková & Pavel Ryšánek, 2022. "Evaluation of the sensitivity of Leptosphaeria maculans isolates causing phoma stem canker in oilseed rape in the Czech Republic to boscalid and dimoxystrobin fungicides," Plant Protection Science, Czech Academy of Agricultural Sciences, vol. 58(4), pages 305-314.
    2. Olufadekemi Fajemisin & Jana Mazáková & Pavel Ryšánek, 2022. "Emergence of Fungicide Sensitivity in Leptosphaeria maculans Isolates Collected from the Czech Republic to DMI Fungicides," Agriculture, MDPI, vol. 12(2), pages 1-13, February.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Olufadekemi Fajemisin & Jana Mazáková & Pavel Ryšánek, 2022. "Evaluation of the sensitivity of Leptosphaeria maculans isolates causing phoma stem canker in oilseed rape in the Czech Republic to boscalid and dimoxystrobin fungicides," Plant Protection Science, Czech Academy of Agricultural Sciences, vol. 58(4), pages 305-314.

    More about this item

    Keywords

    ;
    ;
    ;
    ;

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:gam:jagris:v:15:y:2025:i:15:p:1591-:d:1709171. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: MDPI Indexing Manager (email available below). General contact details of provider: https://www.mdpi.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.