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Establishment and Application of Loop-Mediated Isothermal Amplification Assays for Pathogens of Rice Bakanae Disease

Author

Listed:
  • Xinchun Liu

    (College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, China)

  • Yan Wang

    (College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, China)

  • Yating Zhang

    (College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, China)

  • Jingzhao Xia

    (College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, China)

  • Chenxi Liu

    (College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, China)

  • Yu Song

    (College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, China)

  • Tao Han

    (College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, China
    Donggang City Demonstration and Reproduction Farm, Dandong 118322, China)

  • Songhong Wei

    (College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, China)

  • Wenjing Zheng

    (Liaoning Rice Research Institute, Shenyang 110101, China)

Abstract

Rice bakanae disease (RBD), a major threat in rice-cropping nations, can reduce rice yield and quality. As it is a seed-borne disease, effective seed detection is crucial. Loop-mediated isothermal amplification (LAMP) can rapidly and specifically amplify DNA at a constant temperature with high sensitivity. This research uses LAMP to develop rapid RBD pathogen detection systems. Primers were designed targeting the NRPS31 gene of Fusarium fujikuroi and conserved TEF1α sequences of Fusarium asiaticum , Fusarium proliferatum , and Fusarium andiyazi . These reactions at 60 °C for 60 min had a detection limit of 100 pg·μL −1 , and LAMP proved applicable in field trials.

Suggested Citation

  • Xinchun Liu & Yan Wang & Yating Zhang & Jingzhao Xia & Chenxi Liu & Yu Song & Tao Han & Songhong Wei & Wenjing Zheng, 2025. "Establishment and Application of Loop-Mediated Isothermal Amplification Assays for Pathogens of Rice Bakanae Disease," Agriculture, MDPI, vol. 15(12), pages 1-15, June.
    • Handle: RePEc:gam:jagris:v:15:y:2025:i:12:p:1319-:d:1683004
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