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Potential of Limestonevirus Bacteriophages for Ecological Control of Dickeya solani Causing Bacterial Potato Blackleg

Author

Listed:
  • Martin Kmoch

    (Laboratory of Virology, Department of Genetic Resources, Potato Research Institute Havlíčkův Brod, Dobrovského 2366, 580 01 Havlíčkův Brod, Czech Republic)

  • Josef Vacek

    (Department of Growing Technologies, Potato Research Institute Havlíčkův Brod, Dobrovského 2366, 580 01 Havlíčkův Brod, Czech Republic)

  • Věra Loubová

    (Laboratory of Virology, Department of Genetic Resources, Potato Research Institute Havlíčkův Brod, Dobrovského 2366, 580 01 Havlíčkův Brod, Czech Republic)

  • Karel Petrzik

    (Institute of Molecular Biology, Biology Centre of the Czech Academy of Sciences, Branišovská 1160/31, 370 05 České Budějovice, Czech Republic)

  • Sára Brázdová

    (Institute of Molecular Biology, Biology Centre of the Czech Academy of Sciences, Branišovská 1160/31, 370 05 České Budějovice, Czech Republic)

  • Rudolf Ševčík

    (Institute of Food Preservation, Faculty of Food and Biochemical Technology, University of Chemistry and Technology (VŠCHT), Technická 3, Prague 6—Dejvice, 166 28 Prague, Czech Republic)

Abstract

Pectinolytic bacteria of the family Enterobacteriaceae , specifically Dickeya solani , are known to cause potato blackleg. This study aimed to evaluate the effectiveness of a mixture of two bacteriophages from the genus Limestonevirus in controlling Dickeya solani in both greenhouse and field trials. The potential of bacteriophages for ecological potato control was also assessed. The phages φDs3CZ and φDs20CZ were isolated from soil in the Czech Republic between 2019 and 2021. They were applied preventively and curatively as a solution on artificially wounded and inoculated seed tubers immediately prior to planting. The phage-treated variant showed a highly significant reduction in the extent of D. solani infection compared to the untreated control in both the greenhouse and field trial. The effect of the phages depended on the concentration of the solution, the rate of tuber injury, and the sequence of application. When applied preventively, the phages caused a significantly higher reduction in the rate of blackleg symptoms (86.7% and/or 87.1%) compared to the curative application (54.6 and/or 36.6%). Phages φDs3CZ and φDs20CZ showed potential for use in biological potato control against Dickeya solani .

Suggested Citation

  • Martin Kmoch & Josef Vacek & Věra Loubová & Karel Petrzik & Sára Brázdová & Rudolf Ševčík, 2024. "Potential of Limestonevirus Bacteriophages for Ecological Control of Dickeya solani Causing Bacterial Potato Blackleg," Agriculture, MDPI, vol. 14(3), pages 1-13, March.
    • Handle: RePEc:gam:jagris:v:14:y:2024:i:3:p:497-:d:1359728
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