IDEAS home Printed from https://ideas.repec.org/a/gam/jagris/v13y2023i2p372-d1057274.html
   My bibliography  Save this article

Backcrossing with Marker Assistance to Introduce Broad-Spectrum Bacterial Leaf Blight Resistance in the Malaysian Elite Variety MR297

Author

Listed:
  • Saba Jasim Aljumaili

    (Laboratory of Climate-Smart Food Crop Production, Institute of Tropical Agriculture and Food Security, Universiti Putra Malaysia (UPM), Serdang 43400, Malaysia)

  • Mohd Y. Rafii

    (Laboratory of Climate-Smart Food Crop Production, Institute of Tropical Agriculture and Food Security, Universiti Putra Malaysia (UPM), Serdang 43400, Malaysia
    Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia (UPM), Serdang 43400, Malaysia)

  • Siti Zaharah Sakimin

    (Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia (UPM), Serdang 43400, Malaysia)

  • Khairulmazmi Ahmad

    (Department of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia (UPM), Serdang 43400, Malaysia)

  • Abdulmajeed Al-Jumaily

    (Department of Signal Theory and Communications, Universidad Carlos III de Madrid, Leganes, 28911 Madrid, Spain)

Abstract

A significant part of marker-assisted backcross breeding (MABB) is recurrent parent genome recovery (RPGR). The purpose of this study was to introduce MABB-mediated resistance genes against numerous bacterial leaf blight (BLB) pathogens. Here, we examine the introgression of four Xoo resistance genes to the high-yielding Malaysian MR297 lineage Xa21, xa13 , xa5 , and Xa4 from the IRBB60 lineage. To accomplish both foreground and background selection, we employed polymorphic functional linked markers to target genes and simple sequence repeat (SSR) markers. We used 83 of the 475 authorized polymorphic microsatellite markers to determine the proportion of RPGR in the chosen lines. According to the data, the RPGR at BC 1 F 1 , BC 2 F 1 , and BC 2 F 2 are 81.94, 92.30, and 95.04%, respectively. Incorporating the four BLB resistance genes into the newly created lines would result in long-lasting and comprehensive protection against BLB. Therefore, a resistant gene may be introduced into a population more quickly with MABB than through conventional breeding. All ten lines tested showed a significant level of resistance to BLB, with three lines displaying very high levels of resistance. Consequently, it was recommended that new lines should be used to produce commercially viable rice varieties.

Suggested Citation

  • Saba Jasim Aljumaili & Mohd Y. Rafii & Siti Zaharah Sakimin & Khairulmazmi Ahmad & Abdulmajeed Al-Jumaily, 2023. "Backcrossing with Marker Assistance to Introduce Broad-Spectrum Bacterial Leaf Blight Resistance in the Malaysian Elite Variety MR297," Agriculture, MDPI, vol. 13(2), pages 1-16, February.
  • Handle: RePEc:gam:jagris:v:13:y:2023:i:2:p:372-:d:1057274
    as

    Download full text from publisher

    File URL: https://www.mdpi.com/2077-0472/13/2/372/pdf
    Download Restriction: no

    File URL: https://www.mdpi.com/2077-0472/13/2/372/
    Download Restriction: no
    ---><---

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:gam:jagris:v:13:y:2023:i:2:p:372-:d:1057274. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: MDPI Indexing Manager (email available below). General contact details of provider: https://www.mdpi.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.