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Bta-miR-125a Regulates Milk-Fat Synthesis by Targeting SAA1 mRNA in Bovine Mammary Epithelial Cells

Author

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  • Xiaogang Cui

    (Key Laboratory of Medical Molecular Cell Biology of Shanxi Province, Institute of Biomedical Sciences, Shanxi University, Taiyuan 030006, China)

  • Tianqi Yuan

    (Key Laboratory of Medical Molecular Cell Biology of Shanxi Province, Institute of Biomedical Sciences, Shanxi University, Taiyuan 030006, China)

  • Zhengyu Fang

    (Key Laboratory of Medical Molecular Cell Biology of Shanxi Province, Institute of Biomedical Sciences, Shanxi University, Taiyuan 030006, China)

  • Jiao Feng

    (Key Laboratory of Medical Molecular Cell Biology of Shanxi Province, Institute of Biomedical Sciences, Shanxi University, Taiyuan 030006, China)

  • Changxin Wu

    (Key Laboratory of Medical Molecular Cell Biology of Shanxi Province, Institute of Biomedical Sciences, Shanxi University, Taiyuan 030006, China)

Abstract

The nutritional value of cow milk mainly depends on its fatty acid content and protein composition. The identification of genes controlling milk production traits and their regulatory mechanisms is particularly important for accelerating genetic progress in the breeding of dairy cows. On the basis of mammary gland transcriptome analyses, in this study we identified an miRNA, bta-miR-125a, that could control bovine milk-fat production by targeting the 3′ untranslated region (UTR) of the serum amyloid A-1 (SAA1) mRNA. The presence of synthetic bta-miR-125a (i.e., an miR-125a mimic) significantly down-regulated the expression of luciferase from mRNAs containing the binding sequence for bta-miR-125a in the 3′-UTR in a dual-luciferase reporter assay. Furthermore, the presence of the miR-125a mimic decreased the steady-state level of the SAA1 protein, but increased the accumulation of triglycerides and cholesterol content in bovine mammary epithelial cells (MAC-Ts). Blocking the function of bta-miR-125a using a specific inhibitor decreased the level of triglycerides and cholesterol content in the cells. These results indicate that bta-miR-125a can serve as a positive regulator of lipid synthesis in mammary epithelial cells, which acts by targeting SAA1 gene expression.

Suggested Citation

  • Xiaogang Cui & Tianqi Yuan & Zhengyu Fang & Jiao Feng & Changxin Wu, 2022. "Bta-miR-125a Regulates Milk-Fat Synthesis by Targeting SAA1 mRNA in Bovine Mammary Epithelial Cells," Agriculture, MDPI, vol. 12(3), pages 1-14, February.
    • Handle: RePEc:gam:jagris:v:12:y:2022:i:3:p:344-:d:760715
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    1. Isabelle Jéru & Hasmik Hayrapetyan & Philippe Duquesnoy & Emmanuelle Cochet & Jean-Louis Serre & Josué Feingold & Gilles Grateau & Tamara Sarkisian & Marc Jeanpierre & Serge Amselem, 2009. "Involvement of the Modifier Gene of a Human Mendelian Disorder in a Negative Selection Process," PLOS ONE, Public Library of Science, vol. 4(10), pages 1-5, October.
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