IDEAS home Printed from https://ideas.repec.org/a/gam/jagris/v12y2022i11p1858-d964201.html
   My bibliography  Save this article

A Single Nucleotide Polymorphism in the WIF1 Promoter Region Regulates the Wool Length in Rabbits

Author

Listed:
  • Jiawei Cai

    (College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China)

  • Bohao Zhao

    (College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China)

  • Jiali Li

    (College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China)

  • Zhiyuan Bao

    (College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China)

  • Yang Chen

    (College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China)

  • Yan Liu

    (Zhejiang Academy of Agricultural Sciences, Hangzhou 310022, China)

  • Xinsheng Wu

    (College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China
    Joint International Research Laboratory of Agriculture & Agri-Product Safety, Yangzhou University, Yangzhou 225009, China)

Abstract

Wool length is an important trait in the rabbit wool industry. Wnt inhibitory factor 1 ( WIF1 ), a key gene, regulates hair follicle growth and development. This study explores the WIF1 gene relationship with wool production traits in three different rabbit breeds, Rex rabbit, New Zealand rabbit, and Angora rabbit. The rabbit WIF1 gene sequence was obtained from GenBank, and PCR and Sanger sequencing were applied to screen SNP (single nucleotide polymorphism) from the gene exons and promoter regions. Sequencing results found no SNP in the PCR products of 10 exons from the rabbit WIF1 gene, indicating that the WIF1 gene was not polymorphic. Thus, the gene was conserved in all three rabbit breeds. Only one SNP was found in the WIF1 promoter region, associated with wool-producing traits in rabbits. Luciferase activity assays showed that the AA genotype had higher transcriptional activities than the AG or GG genotype ( p < 0.01). In addition, WIF1 was significantly upregulated in the Rex rabbit compared to the New Zealand rabbit and Angora rabbit ( p < 0.01). In summary, a key SNP in the WIF1 promoter region was found to be associated with rabbit fiber length trait.

Suggested Citation

  • Jiawei Cai & Bohao Zhao & Jiali Li & Zhiyuan Bao & Yang Chen & Yan Liu & Xinsheng Wu, 2022. "A Single Nucleotide Polymorphism in the WIF1 Promoter Region Regulates the Wool Length in Rabbits," Agriculture, MDPI, vol. 12(11), pages 1-9, November.
    • Handle: RePEc:gam:jagris:v:12:y:2022:i:11:p:1858-:d:964201
    as

    Download full text from publisher

    File URL: https://www.mdpi.com/2077-0472/12/11/1858/pdf
    Download Restriction: no
    File URL: https://www.mdpi.com/2077-0472/12/11/1858/
    Download Restriction: no
    ---><---

    References listed on IDEAS

    as
    1. Jen-Chih Hsieh & Laurent Kodjabachian & Martha L. Rebbert & Amir Rattner & Philip M. Smallwood & Cynthia Harryman Samos & Roel Nusse & Igor B. Dawid & Jeremy Nathans, 1999. "A new secreted protein that binds to Wnt proteins and inhibits their activites," Nature, Nature, vol. 398(6726), pages 431-436, April.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Hui Sun & Hui Li & Jie Yan & Xiangdong Wang & Mengyuan Xu & Mingxia Wang & Baozhen Fan & Jieying Liu & Ninghua Lin & Xin Wang & Li Li & Shengtian Zhao & Yongfeng Gong, 2022. "Loss of CLDN5 in podocytes deregulates WIF1 to activate WNT signaling and contributes to kidney disease," Nature Communications, Nature, vol. 13(1), pages 1-18, December.

    More about this item

    Keywords

    WIF1 gene; rabbit; fiber length; SNP; promoter activity;
    All these keywords.

    JEL classification:

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:gam:jagris:v:12:y:2022:i:11:p:1858-:d:964201. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: MDPI Indexing Manager (email available below). General contact details of provider: https://www.mdpi.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.