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In Vitro Evaluation and Genome Mining of Bacillus subtilis Strain RS10 Reveals Its Biocontrol and Plant Growth-Promoting Potential

Author

Listed:
  • Sajid Iqbal

    (Department of Industrial Biotechnology, Atta-ur-Rahman School of Applied Biosciences (ASAB), National University of Sciences and Technology (NUST), H-12, Islamabad 44000, Pakistan)

  • Nimat Ullah

    (Department of Industrial Biotechnology, Atta-ur-Rahman School of Applied Biosciences (ASAB), National University of Sciences and Technology (NUST), H-12, Islamabad 44000, Pakistan)

  • Hussnain Ahmed Janjua

    (Department of Industrial Biotechnology, Atta-ur-Rahman School of Applied Biosciences (ASAB), National University of Sciences and Technology (NUST), H-12, Islamabad 44000, Pakistan)

Abstract

Recently, crop management has involved excessive use of chemical fertilizers and pesticides, compromising public health and environmental integrity. Rhizobacteria, which can enhance plant growth and protect plants from phytopathogen, are eco-friendly and have been attracting increasing attention. In the current study, Bacillus subtilis RS10 isolated from the rhizosphere region of Cynodon dactylon , inhibited the growth of indicator strains and exhibited in vitro plant growth-promoting traits. A whole-genome analysis identified numerous biosynthetic gene clusters encoding antibacterial and antifungal metabolites including bacillibactin, bogorol A, fengycin, bacteriocin, type III polyketides (PKs), and bacilysin. The plant growth-promoting conferring genes involved in nitrogen metabolism, phosphate solubilization, hydrogen sulfide, phytohormones, siderophore biosynthesis, chemotaxis and motility, plant root colonization, lytic enzymes, and biofilm formation were determined. Furthermore, genes associated with abiotic stresses such as high salinity and osmotic stress were identified. A comparative genome analysis indicated open pan-genome and the strain was identified as a novel sequence type (ST-176). In addition, several horizontal gene transfer events were found which putatively play a vital role in the evolution and new functionalities of a strain. In conclusion, the current study demonstrates the potential of RS10 antagonism against important pathogens and plant growth promotion, highlighting its application in sustainable agriculture.

Suggested Citation

  • Sajid Iqbal & Nimat Ullah & Hussnain Ahmed Janjua, 2021. "In Vitro Evaluation and Genome Mining of Bacillus subtilis Strain RS10 Reveals Its Biocontrol and Plant Growth-Promoting Potential," Agriculture, MDPI, vol. 11(12), pages 1-17, December.
    • Handle: RePEc:gam:jagris:v:11:y:2021:i:12:p:1273-:d:702736
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    Citations

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    Cited by:

    1. Tibor Szili-Kovács & Tünde Takács, 2023. "Advanced Research of Rhizosphere Microbial Activity," Agriculture, MDPI, vol. 13(4), pages 1-4, April.
    2. Xiaofei Feng & Rong Xu & Ning Zhao & Dongmei Wang & Mengren Cun & Bin Yang, 2022. "Isolation, Identification, and Characterization of Endophytic Bacillus from Walnut ( Juglans sigillata ) Root and Its Biocontrol Effects on Walnut Anthracnose," Agriculture, MDPI, vol. 12(12), pages 1-18, December.

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