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Energy efficient electric field-assisted green extraction of yeast oil for advanced biodiesel production

Author

Listed:
  • Raspolli Galletti, Anna Maria
  • Di Fidio, Nicola
  • Tozzi, Federico
  • Antonetti, Claudia
  • Caposciutti, Gianluca
  • Bandini, Gabriele
  • Marracci, Mirko
  • Tellini, Bernardo

Abstract

Single Cell Oil (SCO) is a new generation oil for industrial applications aiming at replacing edible oils as feedstock for biofuels and chemicals. One of the main bottlenecks for the scale-up of SCO production is related to the cost and environmental impact of traditional methods for extracting SCO from yeast cells. This study developed a laboratory-scale apparatus for the pretreatment of Lipomyces starkeyi cells to improve lipids extraction. The process adopts a low power and low voltage electric field treatment device for permeabilising the cell's membrane before the lipids recovery by solvent extraction using the bio-based 2-methytetrahydrofuran. Under the optimised process conditions (10 V/cm, 2 min), the recovered SCO cell content (30.0 wt%) was equal to that achieved with the standard acid-catalysed protocol. The extracted SCO was characterised by infrared spectroscopy and used to produce advanced biodiesel with a yield of 84 wt%. The FAMEs profile and the physico-chemical properties of this new generation biofuel were determined and compared to standard biodiesel (EN 14214 and ASTM D6751). With a particular focus on the production of next-generation biodiesel, this novel process has significant potential to replace conventional cell treatments, offering improved efficiency and sustainability.

Suggested Citation

  • Raspolli Galletti, Anna Maria & Di Fidio, Nicola & Tozzi, Federico & Antonetti, Claudia & Caposciutti, Gianluca & Bandini, Gabriele & Marracci, Mirko & Tellini, Bernardo, 2026. "Energy efficient electric field-assisted green extraction of yeast oil for advanced biodiesel production," Renewable Energy, Elsevier, vol. 263(C).
    • Handle: RePEc:eee:renene:v:263:y:2026:i:c:s0960148126003733
    DOI: 10.1016/j.renene.2026.125548
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