IDEAS home Printed from https://ideas.repec.org/a/eee/renene/v103y2017icp383-387.html
   My bibliography  Save this article

Synthesis, characterization and optimization of a two-step immobilized lipase

Author

Listed:
  • Zhao, Kang
  • Cao, Xi
  • Di, Qinjian
  • Wang, Meng
  • Cao, Hao
  • Deng, Li
  • Liu, Junfeng
  • Wang, Fang
  • Tan, Tianwei

Abstract

Lipase (Candida sp. 99–125) was immobilized by a two-step process, adsorption and subsequent entrapment. The formation mechanism of immobilized lipase was investigated by SEM and EDS. With the support of diatomite, the internal network of immobilized lipase showed more porosity and was fully developed. The dimension of the network was more uniform in the present of gelatine. The maximum biodiesel yield was 92% at 40 °C in a 24 h reaction. The biodiesel yield was maintained at about 70% after 11 repeated batch reactions. This enzyme immobilization method has potential applications in biodiesel and other lipase catalyzed synthesis process.

Suggested Citation

  • Zhao, Kang & Cao, Xi & Di, Qinjian & Wang, Meng & Cao, Hao & Deng, Li & Liu, Junfeng & Wang, Fang & Tan, Tianwei, 2017. "Synthesis, characterization and optimization of a two-step immobilized lipase," Renewable Energy, Elsevier, vol. 103(C), pages 383-387.
    • Handle: RePEc:eee:renene:v:103:y:2017:i:c:p:383-387
    DOI: 10.1016/j.renene.2016.11.035
    as

    Download full text from publisher

    File URL: http://www.sciencedirect.com/science/article/pii/S0960148116310047
    Download Restriction: Full text for ScienceDirect subscribers only
    File URL: https://libkey.io/10.1016/j.renene.2016.11.035?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Sun, Shangde & Li, Kaiyue, 2020. "Biodiesel production from phoenix tree seed oil catalyzed by liquid lipozyme TL100L," Renewable Energy, Elsevier, vol. 151(C), pages 152-160.
    2. Oliveira, Anne Caroline Defranceschi & Frensch, Gustavo & Marques, Francisco de Assis & Vargas, José Viriato Coelho & Rodrigues, Maria Luiza Fernandes & Mariano, André Bellin, 2020. "Production of methyl oleate by direct addition of fermented solid Penicillium sumatrense and Aspergillus fumigatus," Renewable Energy, Elsevier, vol. 162(C), pages 1132-1139.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:eee:renene:v:103:y:2017:i:c:p:383-387. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Catherine Liu (email available below). General contact details of provider: http://www.journals.elsevier.com/renewable-energy .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.