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A mixed infection of Mycobacterium avium subsp. paratuberculosis and M. a. hominissuis in one red deer (Cervus elaphus) studied by IS900 BstEII and IS1245 PvuII RFLP analyses: a case report

Author

Listed:
  • M. Moravkova

    (Veterinary Research Institute, Brno, Czech Republic)

  • I. Trcka

    (Veterinary Research Institute, Brno, Czech Republic)

  • J. Lamka

    (Faculty of Pharmacy, Charles University, Hradec Kralove, Czech Republic)

  • I. Pavlik

    (Veterinary Research Institute, Brno, Czech Republic)

Abstract

A mixed infection with Mycobacterium avium subsp. paratuberculosis (MAP) and Mycobacterium avium subsp. hominissuis (MAH) in one naturally infected red deer stag from a game park is described. The animal was euthanized because of symptoms of poor condition, weight loss and chronic diarrhoea. In spite of that, pathological lesions were observed only in the mesenteric lymph nodes, which were five to ten times enlarged with confluent caseous granulomas of 1 to 10 mm in size. Mycobacteria were isolated from all studied samples: a mixed infection of MAP and MAH was confirmed by multiplex PCR for the detection of IS 900, IS9011, IS1245 and dnaJ. MAP of the identical IS900 BstEII RFLP type C1 was isolated from all tissue samples and faeces. MAH isolates were detected in six examined tissue samples, including three mesenteric lymph nodes with caseous granulomas. Only minor differences in the band numbers and position of four different IS1245 PvuII RFLP patterns of MAH isolates were found. It follows from these results that red deer may potentially be infected with MAH, when a MAP infection is under way.

Suggested Citation

  • M. Moravkova & I. Trcka & J. Lamka & I. Pavlik, 2008. "A mixed infection of Mycobacterium avium subsp. paratuberculosis and M. a. hominissuis in one red deer (Cervus elaphus) studied by IS900 BstEII and IS1245 PvuII RFLP analyses: a case report," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 53(8), pages 445-451.
    • Handle: RePEc:caa:jnlvet:v:53:y:2008:i:8:id:1927-vetmed
    DOI: 10.17221/1927-VETMED
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    References listed on IDEAS

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    1. W.y Ayele & M. Macháčková & I. Pavlík, 2001. "The transmission and impact of paratuberculosis infection in domestic and wild ruminants," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 46(7-8), pages 205-224.
    2. I. Pavlik & L. Matlova & L. Dvorska & J.E. Shitaye & I. Parmova, 2005. "Mycobacterial infections in cattle and pigs caused by Mycobacterium aviumcomplex members and atypical mycobacteria in theCzech Republicduring 2000-2004," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 50(7), pages 281-290.
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    Cited by:

    1. M. Blahutkova & P. Fictum & M. Skoric & B. Bezdekova & P. Jahn & P. Kriz & V. Mrlik & I. Slana & M. Kaevska & I. Pavlik, 2011. "Mycobacterium avium subsp. hominissuis infection in two sibling Fjord horses diagnosed using quantitative real time PCR: a case report," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 56(6), pages 294-301.

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