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Pathogen causing Phalaenopsis soft rot disease - 16S rDNA and virulence characterisation

Author

Listed:
  • Sudarsono Sudarsono

    (PMB Lab., Department of Agronomy and Horticulture, Faculty of Agriculture, Bogor Agricultural University, Jl. Meranti ? Darmaga Campus, Bogor, West Java, Indonesia
    PBT Program, IPB Graduate School, Bogor Agricultural University, Jl. Meranti ? Darmaga Campus, Bogor, West Java, Indonesia)

  • Juanita Elina

    (PMB Lab., Department of Agronomy and Horticulture, Faculty of Agriculture, Bogor Agricultural University, Jl. Meranti ? Darmaga Campus, Bogor, West Java, Indonesia
    PBT Program, IPB Graduate School, Bogor Agricultural University, Jl. Meranti ? Darmaga Campus, Bogor, West Java, Indonesia)

  • Giyanto

    (Department of Plant Protection, Bogor Agricultural University, Jl. Meranti ? Darmaga Campus, Bogor, West Java, Indonesia)

  • Dewi Sukma

    (PBT Program, IPB Graduate School, Bogor Agricultural University, Jl. Meranti ? Darmaga Campus, Bogor, West Java, Indonesia)

Abstract

The pathogen causing Phalaenopsis soft rot disease and developed detached leaf inoculation methods were identified. Based on its 16S rDNA sequences, the pathogen causing soft rot disease in Phalaenopsis was Erwinia chrysanthemi/Dickeya chrysanthemi. Both virulent and avirulent strains were revealed. The detached leaf inoculation assay for E. chrysanthemi/D. chrysanthemi resistance evaluation included wounding and inoculating the detached leaf with 108 CFU/ml of bacteria. Soft rot disease symptoms in the inoculated detached leaf were measurable at 20 h after inoculation. The detached leaf assay was applicable for evaluating Phalaenopsis germplasm and progeny resistance in Phalaenopsis breeding programs.

Suggested Citation

  • Sudarsono Sudarsono & Juanita Elina & Giyanto & Dewi Sukma, 2018. "Pathogen causing Phalaenopsis soft rot disease - 16S rDNA and virulence characterisation," Plant Protection Science, Czech Academy of Agricultural Sciences, vol. 54(1), pages 1-8.
    • Handle: RePEc:caa:jnlpps:v:54:y:2018:i:1:id:18-2017-pps
    DOI: 10.17221/18/2017-PPS
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    References listed on IDEAS

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    1. Eric Lam & Naohiro Kato & Michael Lawton, 2001. "Programmed cell death, mitochondria and the plant hypersensitive response," Nature, Nature, vol. 411(6839), pages 848-853, June.
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