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Evaluation of reference genes for gene expression studies in pig muscle tissue by real-time PCR

Author

Listed:
  • M. Nesvadbová

    (Department of Animal Morphology, Physiology and Genetics, Mendel University in Brno, Brno, Czech Republic)

  • A. Knoll

    (Department of Animal Morphology, Physiology and Genetics, Mendel University in Brno, Brno, Czech Republic)

Abstract

The selection of reference genes is essential for gene expression studies when using a real-time quantitative polymerase chain reaction (PCR). Reference gene selection should be performed for each experiment because the gene expression level may be changed in different experimental conditions. In this study, the stability of mRNA expression was determined for seven genes: HPRT1, RPS18, NACA, TBP, TAF4B, RPL32 and OAZ1. The stability of these reference genes was investigated in the skeletal muscle tissue of pig foetuses, piglets and adult pigs using real-time quantitative PCR and SYBR green chemistry. The expression of stability of the used reference genes was calculated using the geNorm application. Different gene expression profiles among the age categories of pigs were found out. RPS18 has been identified as the gene with the most stable expression in the muscle tissue of all pig age categories. HPRT1 and RPL32 were found to have the highest stability in piglets and adult pigs, and in foetuses and adults pigs, respectively. The newly used reference gene, TAF4B, reached the highest expression stability in piglets.

Suggested Citation

  • M. Nesvadbová & A. Knoll, 2011. "Evaluation of reference genes for gene expression studies in pig muscle tissue by real-time PCR," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 56(5), pages 213-216.
    • Handle: RePEc:caa:jnlcjs:v:56:y:2011:i:5:id:1428-cjas
    DOI: 10.17221/1428-CJAS
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    References listed on IDEAS

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    1. Z. Vykoukalová & A. Knoll & S. Čepica, 2009. "Porcine perilipin (PLIN) gene: Structure, polymorphism and association study in Large White pigs," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 54(8), pages 359-364.
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