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Porcine perilipin (PLIN) gene: Structure, polymorphism and association study in Large White pigs

Author

Listed:
  • Z. Vykoukalová

    (Department of Animal Morphology, Physiology and Genetics, Mendel University of Agriculture and Forestry Brno, Brno, Czech Republic)

  • A. Knoll

    (Department of Animal Morphology, Physiology and Genetics, Mendel University of Agriculture and Forestry Brno, Brno, Czech Republic
    Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, Liběchov, Czech Republic)

  • S. Čepica

    (Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, Liběchov, Czech Republic)

Abstract

Perilipin, encoded by the PLIN gene, is a lipid droplet-associated phosphoprotein that functions as a key regulator of triacylglycerol storage and hydrolysis in adipocytes. In this study, structure and variability of the porcine PLIN gene were characterised. PCR fragments encompassing exons 1 to 9 and interspersed introns were sequenced and the obtained sequence was deposited in EMBL/GenBank/DDBJ databases (AM931171). Seven silent polymorphisms and one nonsynonymous polymorphism were detected in the coding sequence. The nonsynonymous polymorphism g.627G>A causing an amino acid substitution p.Val3Ile was found only in Meishan and Meishan × Piétrain cross. Associations were studied between loci g.4119A>G and g.7966T>C, and average daily gain (ADG), backfat thickness (BFT) or lean meat content (LMC) in 166 gilts from two herds. Due to supposed complete linkage disequilibrium between loci g.4119A>G and g.7966T>C only genotype combinations AA-TT, AG-TC and GG-CC were detected. A significant difference (P = 0.0290) between GG-CC and AG-TC genotype combinations for BFT in herd 2 and a suggestive difference (P = 0.0516) between GG-CC and AA-TT genotypes for ADG in herd 1 were detected.

Suggested Citation

  • Z. Vykoukalová & A. Knoll & S. Čepica, 2009. "Porcine perilipin (PLIN) gene: Structure, polymorphism and association study in Large White pigs," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 54(8), pages 359-364.
    • Handle: RePEc:caa:jnlcjs:v:54:y:2009:i:8:id:1661-cjas
    DOI: 10.17221/1661-CJAS
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    Cited by:

    1. M. Nesvadbová & A. Knoll, 2011. "Evaluation of reference genes for gene expression studies in pig muscle tissue by real-time PCR," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 56(5), pages 213-216.

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