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Evaluation of 11 microsatellite loci for their use in paternity testing in Yugoslav Pied cattle (YU Simmental cattle)

Author

Listed:
  • J. Stevanovic

    (Department of Biology, Faculty of Veterinary Medicine, University of Belgrade, Belgrade, Serbia)

  • Z. Stanimirovic

    (Department of Biology, Faculty of Veterinary Medicine, University of Belgrade, Belgrade, Serbia)

  • V. Dimitrijevic

    (Department of Animal Breeding and Genetics, Faculty of Veterinary Medicine, University of Belgrade, Belgrade, Serbia)

  • M. Maletic

    (Department of Biology, Faculty of Veterinary Medicine, University of Belgrade, Belgrade, Serbia)

Abstract

Eleven microsatellite loci (TGLA227, BM2113, TGLA53, ETH10, SPS115, TGLA126, TGLA122, INRA023, ETH3, ETH225, BM1824) were evaluated for their use in paternity testing in the Yugoslav Pied cattle (YU Simmental cattle) population in Serbia. A total of 40 animals were tested. At the 11 tested loci, a total of 91 alleles were detected. The mean number of alleles per locus was 8.273. Polymorphism information content (PIC) values ranged from 0.58 to 0.88 with the mean value of 0.72. The most informative loci were: TGLA53 (14 alleles, PIC = 0.88), TGLA227 (11 alleles, PIC = 0.82), INRA023 (11 alleles, PIC = 0.86), BM2113 (9 alleles, PIC = 0.80). Combined power of discrimination (CPD) for the 11 microsatellite loci was 0.999. The results of the present study confirm that the analysed set of 11 microsatellite markers recommended by ISAG is suitable for paternity testing in Yugoslav Pied cattle in Serbia.

Suggested Citation

  • J. Stevanovic & Z. Stanimirovic & V. Dimitrijevic & M. Maletic, 2010. "Evaluation of 11 microsatellite loci for their use in paternity testing in Yugoslav Pied cattle (YU Simmental cattle)," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 55(6), pages 221-226.
    • Handle: RePEc:caa:jnlcjs:v:55:y:2010:i:6:id:183-2009-cjas
    DOI: 10.17221/183/2009-CJAS
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    1. J. Kučerová & A. Matějíček & O.M. Jandurová & P. Sørensen & E. Němcová & M. Štípková & T. Kott & J. Bouška & J. Frelich, 2006. "Milk protein genes CSN1S1, CSN2, CSN3, LGB and their relation to genetic values of milk production parameters in Czech Fleckvieh," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 51(6), pages 241-247.
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