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The determination of N-methylcarbamate pesticides using enzyme immunoassays with chemiluminescent detection

Author

Listed:
  • B. Mičková

    (Department of Biochemistry and Microbiology, Institute of Chemical Technology, Prague, Czech Republic)

  • P. Rauch

    (Department of Biochemistry and Microbiology, Institute of Chemical Technology, Prague, Czech Republic)

  • A. Montoya

    (Department of Biochemistry and Microbiology, Institute of Chemical Technology, Prague, Czech Republic)

  • E. Ferri

    (Department of Biochemistry and Microbiology, Institute of Chemical Technology, Prague, Czech Republic)

  • F. Fini

    (Department of Biochemistry and Microbiology, Institute of Chemical Technology, Prague, Czech Republic)

  • S. Girotti

    (Department of Biochemistry and Microbiology, Institute of Chemical Technology, Prague, Czech Republic)

Abstract

In the present work, enzyme-linked immunosorbent assays (ELISAs) with chemiluminescent detection for the determination of carbofuran, carbaryl and methiocarb were developed and the analytical parameters of these assays were compared with those of ELISAs with colorimetric detection. The sensitivity of immunochemical methods was expressed as detection limit, linear working range, and I50 value. In comparison with colorimetric ELISA, the ability of the chemiluminescent reagents to detect lower concentrations of HRP allowed to decrease the optimal antibody and conjugate concentrations and to reach better analytical parameters. The experimental comparison of the analytical performance of the ELISAs was carried out by analysing simply diluted fruit juices, spiked at different concentration levels with the above mentioned pesticides. Recovery values for both ELISAs were around 100% and no matrix effects were observed when fruit juices were diluted 1:20 or more.

Suggested Citation

  • B. Mičková & P. Rauch & A. Montoya & E. Ferri & F. Fini & S. Girotti, 2004. "The determination of N-methylcarbamate pesticides using enzyme immunoassays with chemiluminescent detection," Czech Journal of Food Sciences, Czech Academy of Agricultural Sciences, vol. 22(SpecialIs), pages 280-282.
    • Handle: RePEc:caa:jnlcjf:v:22:y:2004:i:specialissue:id:10681-cjfs
    DOI: 10.17221/10681-CJFS
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    Cited by:

    1. Pavel Dostálek & Tomáš Brányik, 2005. "Prospects for rapid bioluminescent detection methods in the food industry - a review," Czech Journal of Food Sciences, Czech Academy of Agricultural Sciences, vol. 23(3), pages 85-92.

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