IDEAS home Printed from https://ideas.repec.org/a/ags/aesjfr/405368.html

Phenotypic and Genotypic Characterization of Cronobacter isolated from Powdered Infant Formula Retailed in Nigeria

Author

Listed:
  • Ezeh, Abimbola
  • Aboaba, Olusimbo
  • Murray, Barbara
  • Tall, Ben
  • Smith, Stella

Abstract

Cronobacter is a genus with emerging pathogens that has been associated with life threatening diseases in neonates, infants and immunocompromised adults. Three Cronobacter species were isolated from powdered infant formula retailed in Nigeria. Different methods of phenotypic and genotypic characterization were carried out. All the isolates were identified biochemically by Microscan identification analysis as Enterobacter sakazakii (98.87%). The Vitek MALDI-TOF system identified the isolates as Cronobacter sakazakii. 16S rRNA sequencing identified the isolates as C. sakazakii. In contrast the use of species-specific PCR assays targeting rpoB, and cgcA, helped to identify two of the three strains as C. sakazakii and the last strain was identified as C. malonaticus. Multi locus sequence typing (MLST) analysis was used to identify each strain’s sequence type and the results identified three new sequence types: 303, 304 and 296. C. sakazakii BAA 894 served as a positive control for all the experiments. Biochemical methods and commercial identification systems are not sensitive enough to identify Cronobacter strains to the species level. Molecular methods are needed to confirm the species identity of strains.

Suggested Citation

  • Ezeh, Abimbola & Aboaba, Olusimbo & Murray, Barbara & Tall, Ben & Smith, Stella, 2016. "Phenotypic and Genotypic Characterization of Cronobacter isolated from Powdered Infant Formula Retailed in Nigeria," Journal of Food Research, Canadian Center of Science and Education, vol. 5(4).
    • Handle: RePEc:ags:aesjfr:405368
    as

    Download full text from publisher

    File URL: https://ageconsearch.umn.edu/record/405368/files/60364-217304-1-PB.pdf
    Download Restriction: no
    ---><---

    More about this item

    Keywords

    ;

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:ags:aesjfr:405368. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: AgEcon Search (email available below). General contact details of provider: https://www.ccsenet.org/ .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.