IDEAS home Printed from https://ideas.repec.org/a/ags/aesjfr/405320.html

Increasing the Yield of Soluble Mustard Protein Isolate

Author

Listed:
  • Lorenzo, L.
  • Diosady, Levente

Abstract

The objective of this study was to investigate methods for improving the yield of acid soluble mustard protein isolate (SPI) by solubilizing isoelectrically precipitated protein isolate (PPI). The SPI is more valuable, as it can be used in unique food applications. Four treatments were tested in the acidic pH range: Alcalase hydrolysis; transglutaminase cross-linking; salting in with NaCl, Na5P3O10, and (NaPO3)6; and protective colloid formation with pectin. The effectiveness of each treatment was determined by measuring the increase in nitrogen solubility (AOCS-Ba11-65). Alcalase hydrolysis improved PPI solubility evenly in the 2.5-3.5 pH range, effectively eliminating the solubility minimum near the isoelectric point. At pH 3, the hydrolysis treatment increased solubility from ~20% to a maximum of ~70% (0.04 g of enzyme preparation / g PPI, 2 h, pH 8.5, 50-55oC). Protein hydrolysis during isolate production could increase the yield of SPI from 0.16 to 0.75 kg per kilogram of mustard protein.

Suggested Citation

  • Lorenzo, L. & Diosady, Levente, 2015. "Increasing the Yield of Soluble Mustard Protein Isolate," Journal of Food Research, Canadian Center of Science and Education, vol. 4(6).
    • Handle: RePEc:ags:aesjfr:405320
    as

    Download full text from publisher

    File URL: https://ageconsearch.umn.edu/record/405320/files/54916-189942-1-SM.pdf
    Download Restriction: no
    ---><---

    More about this item

    Keywords

    ;

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:ags:aesjfr:405320. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: AgEcon Search (email available below). General contact details of provider: https://www.ccsenet.org/ .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.