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RNA supply drives physiological granule assembly in neurons

Author

Listed:
  • Karl E. Bauer

    (Ludwig Maximilians University)

  • Niklas Bargenda

    (Ludwig Maximilians University)

  • Rico Schieweck

    (Ludwig Maximilians University)

  • Christin Illig

    (Ludwig Maximilians University)

  • Inmaculada Segura

    (Ludwig Maximilians University
    Max Planck Institute for Biological Intelligence (in foundation))

  • Max Harner

    (Ludwig Maximilians University)

  • Michael A. Kiebler

    (Ludwig Maximilians University)

Abstract

Membraneless cytoplasmic condensates of mRNAs and proteins, known as RNA granules, play pivotal roles in the regulation of mRNA fate. Their maintenance fine-tunes time and location of protein expression, affecting many cellular processes, which require complex protein distribution. Here, we report that RNA granules—monitored by DEAD-Box helicase 6 (DDX6)—disassemble during neuronal maturation both in cell culture and in vivo. This process requires neuronal function, as synaptic inhibition results in reversible granule assembly. Importantly, granule assembly is dependent on the RNA-binding protein Staufen2, known for its role in RNA localization. Altering the levels of free cytoplasmic mRNA reveals that RNA availability facilitates DDX6 granule formation. Specifically depleting RNA from DDX6 granules confirms RNA as an important driver of granule formation. Moreover, RNA is required for DDX6 granule assembly upon synaptic inhibition. Together, this data demonstrates how RNA supply favors RNA granule assembly, which not only impacts subcellular RNA localization but also translation-dependent synaptic plasticity, learning, and memory.

Suggested Citation

  • Karl E. Bauer & Niklas Bargenda & Rico Schieweck & Christin Illig & Inmaculada Segura & Max Harner & Michael A. Kiebler, 2022. "RNA supply drives physiological granule assembly in neurons," Nature Communications, Nature, vol. 13(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-30067-3
    DOI: 10.1038/s41467-022-30067-3
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    References listed on IDEAS

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    Cited by:

    1. Kavya Vinayan Pushpalatha & Mathilde Solyga & Akira Nakamura & Florence Besse, 2022. "RNP components condense into repressive RNP granules in the aging brain," Nature Communications, Nature, vol. 13(1), pages 1-15, December.

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