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Optimization of Cellulase Production by a Novel Endophytic Fungus Penicillium oxalicum R4 Isolated from Taxus cuspidata

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  • Hongkun Li

    (College of Chemistry, Chemical Engineering and Resource Utilization, Northeast Forestry University, Harbin 150040, China
    Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, China)

  • Meijia Dou

    (College of Chemistry, Chemical Engineering and Resource Utilization, Northeast Forestry University, Harbin 150040, China
    Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, China)

  • Xinyu Wang

    (College of Chemistry, Chemical Engineering and Resource Utilization, Northeast Forestry University, Harbin 150040, China
    Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, China)

  • Na Guo

    (College of Chemistry, Chemical Engineering and Resource Utilization, Northeast Forestry University, Harbin 150040, China
    Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, China)

  • Ping Kou

    (College of Chemistry, Chemical Engineering and Resource Utilization, Northeast Forestry University, Harbin 150040, China
    Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, China)

  • Jiao Jiao

    (College of Chemistry, Chemical Engineering and Resource Utilization, Northeast Forestry University, Harbin 150040, China
    Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, China)

  • Yujie Fu

    (College of Chemistry, Chemical Engineering and Resource Utilization, Northeast Forestry University, Harbin 150040, China
    Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, China
    The College of Forestry, Beijing Forestry University, Beijing 100083, China)

Abstract

Endophytic fungi inside a plant can degrade a portion of plant lignin and cellulose. Endophytic Penicillium is one of the industrial microorganisms with the advantage of producing enzymes with a complete enzyme system that can be secreted into the extracellular space. The natural evolution of ancient tree species from special natural geographic environments to screen out cellulase-producing strains with excellent characteristics provides a promising direction for future industrial enzymes. The present study successfully isolated and screened a novel fungal endophyte, Penicillium oxalicum R4, with higher cellulase activity from Taxus cuspidata . Under the optimized culture conditions obtained by a Box–Behnken design (BBD) and an artificial neural network–genetic algorithm (ANN–GA), yields of Filter Paperase (FPase), Carboxymethyl Cellulase (CMCase) and β-glucosidase (βGLase) produced by P. oxalicum R4 were 1.45, 5.27 and 6.35 U/mL, which were approximately 1.60-fold, 1.59-fold and 2.16-fold higher than those of the non-optimized culture, respectively. The discovery of cellulase-producing strains of endophytic fungi located in special natural geographic environments, such as Taxus cuspidata , which is known as a living plant fossil, provides new research directions for future industrial enzymes.

Suggested Citation

  • Hongkun Li & Meijia Dou & Xinyu Wang & Na Guo & Ping Kou & Jiao Jiao & Yujie Fu, 2021. "Optimization of Cellulase Production by a Novel Endophytic Fungus Penicillium oxalicum R4 Isolated from Taxus cuspidata," Sustainability, MDPI, vol. 13(11), pages 1-14, May.
    • Handle: RePEc:gam:jsusta:v:13:y:2021:i:11:p:6006-:d:562872
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    References listed on IDEAS

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    1. Månsson, André & Sanches-Pereira, Alessandro & Hermann, Sebastian, 2014. "Biofuels for road transport: Analysing evolving supply chains in Sweden from an energy security perspective," Applied Energy, Elsevier, vol. 123(C), pages 349-357.
    2. Sarkar, Nibedita & Ghosh, Sumanta Kumar & Bannerjee, Satarupa & Aikat, Kaustav, 2012. "Bioethanol production from agricultural wastes: An overview," Renewable Energy, Elsevier, vol. 37(1), pages 19-27.
    3. Troiano, D. & Orsat, V. & Dumont, M.J., 2020. "Status of filamentous fungi in integrated biorefineries," Renewable and Sustainable Energy Reviews, Elsevier, vol. 117(C).
    4. Moreira, José Roberto & Romeiro, Viviane & Fuss, Sabine & Kraxner, Florian & Pacca, Sérgio A., 2016. "BECCS potential in Brazil: Achieving negative emissions in ethanol and electricity production based on sugar cane bagasse and other residues," Applied Energy, Elsevier, vol. 179(C), pages 55-63.
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