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Rhizomucor miehei Lipase Supported on Inorganic Solids, as Biocatalyst for the Synthesis of Biofuels: Improving the Experimental Conditions by Response Surface Methodology

Author

Listed:
  • Juan Calero

    (Departamento de Química Orgánica/Universidad de Córdoba, Campus de Rabanales, Ed. Marie Curie, 14014 Córdoba, Spain)

  • Diego Luna

    (Departamento de Química Orgánica/Universidad de Córdoba, Campus de Rabanales, Ed. Marie Curie, 14014 Córdoba, Spain)

  • Carlos Luna

    (Departamento de Química Orgánica/Universidad de Córdoba, Campus de Rabanales, Ed. Marie Curie, 14014 Córdoba, Spain)

  • Felipa M. Bautista

    (Departamento de Química Orgánica/Universidad de Córdoba, Campus de Rabanales, Ed. Marie Curie, 14014 Córdoba, Spain)

  • Beatriz Hurtado

    (Departamento de Química Orgánica/Universidad de Córdoba, Campus de Rabanales, Ed. Marie Curie, 14014 Córdoba, Spain)

  • Antonio A. Romero

    (Departamento de Química Orgánica/Universidad de Córdoba, Campus de Rabanales, Ed. Marie Curie, 14014 Córdoba, Spain)

  • Alejandro Posadillo

    (Seneca Green Catalyst S.L., Campus de Rabanales, 14014 Córdoba, Spain)

  • Rafael Estevez

    (Departamento de Química Orgánica/Universidad de Córdoba, Campus de Rabanales, Ed. Marie Curie, 14014 Córdoba, Spain)

Abstract

Two inorganic solids have been evaluated as supports of Lipozyme RM IM, a Rhizomucor miehei lipase immobilized on a macroporous anion exchange resin, in order to improve its application as a biocatalyst in the synthesis of biofuels. The experimental conditions have been optimized to get the selective transesterification of sunflower oil, by using a multi-factorial design based on the response surface methodology (RSM). In this way, the effects of several reaction parameters on the selective ethanolysis of triglycerides to produce Ecodiesel, a biodiesel-like biofuel constitute by one mole of monoglyceride (MG) and two moles of fatty acid ethyl ester (FAEE), have been evaluated. Thus, it was obtained that a 6:1 oil/ethanol molar ratio, 0.215 g of biocatalyst supported in silica-gel (0.015 g Lipase/0.2 g silica-gel), 50 µL of 10 N NaOH, together with previous optimized reaction parameters, 35 °C reaction temperature and 120 min of reaction time, gave the best results (conversions around 70%; selectivity around 65%; kinematic viscosities about 9.3 mm 2 /s) in the reaction studied. Besides, Lipozyme RM IM, supported on silica-gel, biocatalyst exhibited a very good stability, remaining its activity even after 15 cycles.

Suggested Citation

  • Juan Calero & Diego Luna & Carlos Luna & Felipa M. Bautista & Beatriz Hurtado & Antonio A. Romero & Alejandro Posadillo & Rafael Estevez, 2019. "Rhizomucor miehei Lipase Supported on Inorganic Solids, as Biocatalyst for the Synthesis of Biofuels: Improving the Experimental Conditions by Response Surface Methodology," Energies, MDPI, vol. 12(5), pages 1-15, March.
    • Handle: RePEc:gam:jeners:v:12:y:2019:i:5:p:831-:d:210424
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    References listed on IDEAS

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    Cited by:

    1. Rafael Estevez & Laura Aguado-Deblas & Francisco J. López-Tenllado & Carlos Luna & Juan Calero & Antonio A. Romero & Felipa M. Bautista & Diego Luna, 2022. "Biodiesel Is Dead: Long Life to Advanced Biofuels—A Comprehensive Critical Review," Energies, MDPI, vol. 15(9), pages 1-39, April.
    2. Diego Luna & Rafael Estevez, 2022. "Optimization of Biodiesel and Biofuel Process," Energies, MDPI, vol. 15(16), pages 1-4, August.

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