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Optimization of biohydrogen production from microalgae by response surface methodology (RSM)

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  • Nazarpour, Mehrshad
  • Taghizadeh-Alisaraei, Ahmad
  • Asghari, Ali
  • Abbaszadeh-Mayvan, Ahmad
  • Tatari, Aliasghar

Abstract

In the present study, the design and fabrication of a micro-photobioreactor to produce the bio-hydrogen are aimed. Furthermore, the optimization of variables affecting hydrogen production was optimized using the response surface methodology (RSM). A quadratic model was used to predict the behavior of samples. The central composite design was applied using 20 treatments and 6 replications in the central points. Independent variables for evaluation included sulfur concentration (0.5–1%), run time (5–120 h) and algal biomass concentration (50–100 g/L). The results suggested that test length had a significant impact on hydrogen production and that sulfur content and biomass concentration had no significant effect on hydrogen production but did cause a little increase. The experimental values of response variable in these optimal conditions match the predicted values. Optimal conditions to produce bio-hydrogen were identified as the sulfur concentration of 0.75%, run time of 101.96 h, and biomass concentration of 53.31 g/L for maximum production of bio-hydrogen (66.32 mL g-VS−1). In conclusion, the response surface methodology can predict the production and extraction of bio-hydrogen in photobioreactors.

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  • Nazarpour, Mehrshad & Taghizadeh-Alisaraei, Ahmad & Asghari, Ali & Abbaszadeh-Mayvan, Ahmad & Tatari, Aliasghar, 2022. "Optimization of biohydrogen production from microalgae by response surface methodology (RSM)," Energy, Elsevier, vol. 253(C).
  • Handle: RePEc:eee:energy:v:253:y:2022:i:c:s0360544222009628
    DOI: 10.1016/j.energy.2022.124059
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    1. Zhang, Zhiping & Ai, Fuke & Zhang, Haorui & Zhang, Huan & Zhu, Shengnan & Zhang, Quanguo & Li, Yameng, 2023. "Synergetic effect evaluation of light and mass transfer enhancement strategies on photo fermentative biohydrogen production process: Illumination, shake, and high solid level," Energy, Elsevier, vol. 269(C).

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