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Utilisation of wheat bran as a substrate for bioethanol production using recombinant cellulases and amylolytic yeast

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  • Cripwell, Rosemary
  • Favaro, Lorenzo
  • Rose, Shaunita H.
  • Basaglia, Marina
  • Cagnin, Lorenzo
  • Casella, Sergio
  • van Zyl, Willem

Abstract

Wheat bran, generated from the milling of wheat, represents a promising feedstock for the production of bioethanol. This substrate consists of three main components: starch, hemicellulose and cellulose. The optimal conditions for wheat bran hydrolysis have been determined using a recombinant cellulase cocktail (RCC), which contains two cellobiohydrolases, an endoglucanase and a β-glucosidase. The 10% (w/v, expressed in terms of dry matter) substrate loading yielded the most glucose, while the 2% loading gave the best hydrolysis efficiency (degree of saccharification) using unmilled wheat bran. The ethanol production of two industrial amylolytic Saccharomyces cerevisiae strains, MEL2[TLG1-SFA1] and M2n[TLG1-SFA1], were compared in a simultaneous saccharification and fermentation (SSF) for 10% wheat bran loading with or without the supplementation of optimised RCC. The recombinant yeast S. cerevisiae MEL2[TLG1-SFA1] and M2n[TLG1-SFA1] completely hydrolysed wheat bran’s starch producing similar amounts of ethanol (5.3±0.14g/L and 5.0±0.09g/L, respectively). Supplementing SSF with RCC resulted in additional ethanol production of about 2.0g/L. Scanning electron microscopy confirmed the effectiveness of both RCC and engineered amylolytic strains in terms of cellulose and starch depolymerisation.

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  • Cripwell, Rosemary & Favaro, Lorenzo & Rose, Shaunita H. & Basaglia, Marina & Cagnin, Lorenzo & Casella, Sergio & van Zyl, Willem, 2015. "Utilisation of wheat bran as a substrate for bioethanol production using recombinant cellulases and amylolytic yeast," Applied Energy, Elsevier, vol. 160(C), pages 610-617.
    • Handle: RePEc:eee:appene:v:160:y:2015:i:c:p:610-617
    DOI: 10.1016/j.apenergy.2015.09.062
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    1. Caspeta, Luis & Caro-Bermúdez, Mario A. & Ponce-Noyola, Teresa & Martinez, Alfredo, 2014. "Enzymatic hydrolysis at high-solids loadings for the conversion of agave bagasse to fuel ethanol," Applied Energy, Elsevier, vol. 113(C), pages 277-286.
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    2. Zhao, Chen & Zou, Zongsheng & Li, Jisheng & Jia, Honglei & Liesche, Johannes & Fang, Hao & Chen, Shaolin, 2017. "A novel and efficient bioprocess from steam exploded corn stover to ethanol in the context of on-site cellulase production," Energy, Elsevier, vol. 123(C), pages 499-510.
    3. Malherbe, Sarel J.M. & Cripwell, Rosemary A. & Favaro, Lorenzo & van Zyl, Willem H. & Viljoen-Bloom, Marinda, 2023. "Triticale and sorghum as feedstock for bioethanol production via consolidated bioprocessing," Renewable Energy, Elsevier, vol. 206(C), pages 498-505.
    4. Zhang, Qiuzhuo & Huang, Huiqin & Han, Hui & Qiu, Zhen & Achal, Varenyam, 2017. "Stimulatory effect of in-situ detoxification on bioethanol production by rice straw," Energy, Elsevier, vol. 135(C), pages 32-39.
    5. Shah, A.T. & Favaro, L. & Alibardi, L. & Cagnin, L. & Sandon, A. & Cossu, R. & Casella, S. & Basaglia, M., 2016. "Bacillus sp. strains to produce bio-hydrogen from the organic fraction of municipal solid waste," Applied Energy, Elsevier, vol. 176(C), pages 116-124.
    6. Nicoletta Gronchi & Lorenzo Favaro & Lorenzo Cagnin & Silvia Brojanigo & Valentino Pizzocchero & Marina Basaglia & Sergio Casella, 2019. "Novel Yeast Strains for the Efficient Saccharification and Fermentation of Starchy By-Products to Bioethanol," Energies, MDPI, vol. 12(4), pages 1-13, February.
    7. Du, Jiliang & Chen, Le & Li, Jianan & Zuo, Ranan & Yang, Xiushan & Chen, Hongzhang & Zhuang, Xinshu & Tian, Shen, 2018. "High-solids ethanol fermentation with single-stage methane anaerobic digestion for maximizing bioenergy conversion from a C4 grass (Pennisetum purpereum)," Applied Energy, Elsevier, vol. 215(C), pages 437-443.
    8. Zhao, Chen & Zou, Zongsheng & Li, Jisheng & Jia, Honglei & Liesche, Johannes & Chen, Shaolin & Fang, Hao, 2018. "Efficient bioethanol production from sodium hydroxide pretreated corn stover and rice straw in the context of on-site cellulase production," Renewable Energy, Elsevier, vol. 118(C), pages 14-24.
    9. Zaafouri, Kaouther & Ziadi, Manel & ben Hassen-Trabelsi, Aida & Mekni, Sabrine & Aïssi, Balkiss & Alaya, Marwen & Hamdi, Moktar, 2017. "Enzymatic saccharification and liquid state fermentation of hydrothermal pretreated Tunisian Luffa cylindrica (L.) fibers for cellulosic bioethanol production," Renewable Energy, Elsevier, vol. 114(PB), pages 1209-1213.

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