Proceedings: 3rd International Conference on Food and Agricultural Economics: THE IMPACT OF HELMINTHES PARASITES INFECTION ONSERUM PROTEINLES PROFILE OF CATFISH CLARİES LAZERA USING POLYACRYLAMİDE GEL ELECTROPHORESİS

The fresh water fisheries are increasingly important economically to the people living along the banks of the Nile that can maintain sustainability of the resources, so fishes has been a major of source of protein and energy that pointed out by many survey and laboratory investigation (Khalil 1971). The mean sources are contribution in the total fish catch is 82% for the White Nile so freshwater fishes occurring in the Sudan may represented a great potential for the future developing of aquaculture with economical and social consequences further research on fish diseases urgently needed especially the infection of parasites which from about 80% of fish diseases. There were many the economically losses due to fish diseases were manifested in high mortality rate and , unmarketable (FAO, 2005). Screening common Nile fishes for helminthes parasites infection revealed Clarias lazera among the most infected fishes by nematodes and Cestodes. A number of hundred Clarias lazera collected from Jebel Aulia reservoir on the White Nile south of Khartoum (45Km) in 2010-2013 were segregated due to the intensity of infection from high to low when both nematodes and cestodes parasites were present. Polyacrylamide gel electrophoresis for the blood sera was used to investigate the impact of parasite infection on serum proteins profile as indicator to fish health status. These findings can, as well, used to check the principle on immunity to diseases in fishes. Polyacrylamide gel electrophoresis (PAGE) known to provide a high resolution method for fractionation and physical – chemical characterization of molecules based on size conformation, and net charge. Its application in the present study recorded different protein types as indicated by different fractional bands. Accordingly, and following the standard marker of serum protein profile, these recorded fractions detected were classified in four groups namely: albumin (9-57KD), alpha globulin ( ≥57KD,81 KD), Beta globulin (≥81-185 KD) and Gamma globulin, (≥200 KD). The results showed that helminthic parasites affect with reduction in the level of albumin and immune globulins IgM. The recommended more polyacrylamide gel electrophoresis investigation on fish pathogens as research programs are need .