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Isozymes Reveal Genetic Relationships In Banana Germplasm

Author

Listed:
  • Manshardt, R.M.
  • Lebot, V.
  • Aradhya, K.M.

Abstract

Isozyme variation was analyzed in a collection of banana cultivars to clarify genetic relationships among clones and to determine the affinities of Hawaiian bananas of early Polynesian introduction to other cultivated types. Enzymes were extracted from fresh young leaf tissues in modified Bousquet buffer at 4° C and separated by starch gel electrophoresis. Polymorphic enzymes, including ACO, MDH, PGI, and PGM, were well resolved in a continuous histidine citrate (pH 6.5) buffer system after 7 hr of electrophoresis at 15 V/cm. Isozyme variation was analyzed by cluster analysis, and the resulting groups generally corresponded well with the classification of Simmonds (1959) based on morphology. Three groups of Hawaiian cultivars were evident in this study. They were distinct from other clones and corresponded completely with a traditional classification, based on morphology, which distinguished ^Iholena', 'Maia Maoli' and 'Popoulu' groups. Cluster analysis placed the Hawaiian bananas squarely among the AAB cultivars, supporting Simmond's estimation of the genomic constitution of 'Maia Maoli' and 'Popoulu' cultivars, but contradicting his assignment of 'Iholena' bananas to the AAA group.

Suggested Citation

  • Manshardt, R.M. & Lebot, V. & Aradhya, K.M., 1990. "Isozymes Reveal Genetic Relationships In Banana Germplasm," 26th Annual Meeting, July 29 to August 4, 1990, Mayaguez, Puerto Rico 259338, Caribbean Food Crops Society.
    • Handle: RePEc:ags:cfcs90:259338
    DOI: 10.22004/ag.econ.259338
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