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Photochemical Scheme for Reversibly Photoswitchable Fluorescent Proteins Used in Biological Imaging

In: Trends in Biomathematics: Stability and Oscillations in Environmental, Social, and Biological Models

Author

Listed:
  • A. Pellissier-Tanon

    (PSL University, Sorbonne Université, CNRS, Pasteur, Département de chimie, École normale supérieure)

  • R. Chouket

    (PSL University, Sorbonne Université, CNRS, Pasteur, Département de chimie, École normale supérieure)

  • T. Le Saux

    (PSL University, Sorbonne Université, CNRS, Pasteur, Département de chimie, École normale supérieure)

  • L. Jullien

    (PSL University, Sorbonne Université, CNRS, Pasteur, Département de chimie, École normale supérieure)

  • A. Espagne

    (PSL University, Sorbonne Université, CNRS, Pasteur, Département de chimie, École normale supérieure)

  • A. Lemarchand

    (Laboratoire de Physique Théorique de la Matière Condensée, Sorbonne Université, CNRS)

Abstract

The complex kinetics of reversibly photoswitchable fluorescent proteins (RSFPs) is exploited in biological imaging. We analyze the fluorescence evolution of RSFPs submitted to two-color illumination at different light intensities appropriately chosen to change the rate-limiting steps of photoswitching. Starting from a reasonable seven-state photoswitching mechanism, we use the steady-state approximation to obtain simple reduced mechanisms associated with a small number of characteristic times. The comparison with experimental results for the RSFP Dronpa-2 provides previously unknown kinetic information on photoswitching.

Suggested Citation

  • A. Pellissier-Tanon & R. Chouket & T. Le Saux & L. Jullien & A. Espagne & A. Lemarchand, 2022. "Photochemical Scheme for Reversibly Photoswitchable Fluorescent Proteins Used in Biological Imaging," Springer Books, in: Rubem P. Mondaini (ed.), Trends in Biomathematics: Stability and Oscillations in Environmental, Social, and Biological Models, pages 23-36, Springer.
    • Handle: RePEc:spr:sprchp:978-3-031-12515-7_2
    DOI: 10.1007/978-3-031-12515-7_2
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