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Purification of Protease Enzymes from Wild Poinsettia (Euphorbia Heterophylla) Plant with Ammonium Sulphate

In: Proceedings of the 2022 Brawijaya International Conference (BIC 2022)

Author

Listed:
  • R. Amilia Destryana

    (Universitas Wiraraja)

  • Dyah Ayu Fajarianingtyas

    (Universitas Wiraraja)

  • Aryo Wibisono

    (Universitas Wiraraja)

  • Yuli Witono

    (Universitas Jember)

  • Maria Belgis

    (Universitas Jember)

  • Iwan Taruna

    (Universitas Jember)

Abstract

Protease enzymes are one of the essential enzyme groups that have been widely studied and have very high economic value. The study aimed to determine the optimum protease enzyme extraction method from the wild poinsettia (Euphorbia heterophylla) to produce the highest protease enzyme activity. It was a salting-out extraction method with adding ammonium sulphate salt at saturation levels of 50%, 60%, and 70%. The results showed that adding ammonium sulphate at a concentration of 50% is the most effective protease extraction from the latex of wild poinsettia. It showed the highest percentage yield (17.63 ± 4.74%) and the soluble protein content (4.76 ± 0.014%), while the protease enzyme activity was the lowest (2.9 x 103 ± 0.0142 unit/mg). The highest specific protease activity is achieved by extraction with 70% ammonium sulphate, i.e., 1.8 x 104 units/mg, at the saturation level of 50% and 60% ammonium sulphate; the specific activity of the protease wild poinsettia tended to be low. In conclusion, adding ammonium sulphate salt at saturation levels of 50%, 60%, and 70% showed the different characterization of protease enzymes from wild poinsettia plant latex.

Suggested Citation

  • R. Amilia Destryana & Dyah Ayu Fajarianingtyas & Aryo Wibisono & Yuli Witono & Maria Belgis & Iwan Taruna, 2023. "Purification of Protease Enzymes from Wild Poinsettia (Euphorbia Heterophylla) Plant with Ammonium Sulphate," Advances in Economics, Business and Management Research, in: Yusfan Adeputera Yusran & Femiana Gapsari Madhi Fitri & Titin Andri Wihastuti & Fajar Ari Nugroho & (ed.), Proceedings of the 2022 Brawijaya International Conference (BIC 2022), pages 449-456, Springer.
    • Handle: RePEc:spr:advbcp:978-94-6463-140-1_45
    DOI: 10.2991/978-94-6463-140-1_45
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