Isolation of Yeast and Extraction of Superoxide Dismutase and ß Glucanase and Its Application in Agriculture

Enzymes are highly efficient biocatalysts researched for industrial-scale catalysis because of their several distinct advantages. The present research includes the extraction of enzymes that have agricultural applications from yeast. The yeast cells were used to produce enzymes that had agricultural applications. The yeast cells were first isolated from two different curd sources. Isolation was done by serial dilution followed by the spread plate technique was employed for the collected curd samples. The isolates were grown on specific media like Yeast Potato Dextrose (YPD) medium. Cultural, and morphological study was performed for the selected colonies. The isolated yeast cells were screened for the production of enzymes like superoxide dismutase and β Glucanase. These enzymes are Significant and have extensive applications in agriculture. The yeast cells were then grown on YPD broth and incubated at 30°C for 24 h in an orbital shaking incubator at 180 rpm. The cells were centrifuged and lysed with cell-specific buffer for extraction of enzymes. Qualitative and Quantitative analysis was performed on the crude enzyme suspension to understand the activity rate of superoxide dismutase and β glucanase. The main study involves the action of the enzymes, superoxide dismutase and β glucanase on seed germination and plant growth. These enzymes can be used in modern agriculture to gain tolerance to stress conditions and reduce reliance on chemical inputs, making agriculture more eco-friendly.