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Callus Induction and Characterization in Oryza Sativa Indica

In: Convergence of Technology & Biology ─ Transforming Life Sciences

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  • Jaanvi Dayal
  • Velamanchi Vaishnavi
  • Sushma Patkar

Abstract

In rice tissue culture, optimizing plant growth regulators (PGRs) is crucial for effective callus induction. The present study investigates the induction of rice callus in Murashige and Skoog (MS) media supplemented with varying concentrations of 6-Benzylaminopurine (BAP) and Indole-3-acetic acid (IAA), namely, in concentrations of 3:1 ratio, 2:1 ratio and 1:1 ratio respectively. The goal was to identify the most effective PGR combination and concentration that promotes optimal callus growth. Callus fresh and dry weight were measured to assess the growth response under different conditions. Highest mean fresh weight of callus was obtained for 3:1 ratio of BAP to IAA (0.55 ± 0.38 gram). The present study provides valuable insights into optimizing rice tissue culture protocols for improved callus induction, offering potential applications in plant propagation and genetic improvement.

Suggested Citation

  • Jaanvi Dayal & Velamanchi Vaishnavi & Sushma Patkar, 2025. "Callus Induction and Characterization in Oryza Sativa Indica," Convergence of Technology & Biology ─ Transforming Life Sciences, in: Malathi Varma & S.Parijatham Kanchana & G.Sony (ed.),Convergence of Technology & Biology ─ Transforming Life Sciences, chapter 1, pages 1-7, Shanlax Publications.
    • Handle: RePEc:dax:ctbtls:978-93-6163-763-6:p:1-7
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