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Detection Of HBLD Toxin Gene By Bacillus Cereus Isolated From Meat Curry Food Samples In Malaysian Restaurants

Author

Listed:
  • Marwan Msarah

    (Department of Biological Science and Biotechnology, Faculty Science and Technology, University Kebangsaan Malaysia, 43000 UKM, Bangi,Malaysia)

  • Ahmed Alsier

    (Department of Food Sciences, Faculty Science and Technology, University Kebangsaan Malaysia, 43000 UKM, Bangi, Malaysia)

  • Sahilah, A.M

    (Department of Food Sciences, Faculty Science and Technology, University Kebangsaan Malaysia, 43000 UKM, Bangi, Malaysia)

Abstract

Bacillus cereus is a ubiquitous foodborne pathogen, can cause food poisoning, leading to infections, have two major types of food poisoning emetic and diarrheal. Foods rich in protein such as meat are associated with foodborne outbreaks of diarrhea caused by B. cereus. The aim of this study is to isolate and identify B. cereus from ready to eat (RTE) meat curry from restaurants in Malaysia and to detect hblD pathogenic gene of B. cereus isolates. Mannitol egg yolk polymyxin agar was used as a selective isolation medium. Commercially available kits and boiling methods were used for DNA extraction, samples acquired from restaurants were examined for the presence of Hemolysin BL gene by polymerase chain reaction (PCR). Among all isolates, twenty-four of B. cereus isolates detected for HBL enterotoxin production by the discontinuous pattern on HBL sheep blood agar then confirmed by biochemical tests. More than 58.33 % of the isolate showed discontinuous hemolysis pattern on HBl blood agar and 29.16% of the samples were shown positive for hblD gene that can cause diarrhea with the size of 807bp on gel. This study demonstrated that RTE meat curry was a potential source for entero-toxigenic B. cereus and the presence of the hblD toxin genes for the HBL complex in the isolates tested were highly associated. Therefore, these meat curry isolates should be regarded as potential toxin producers.

Suggested Citation

  • Marwan Msarah & Ahmed Alsier & Sahilah, A.M, 2020. "Detection Of HBLD Toxin Gene By Bacillus Cereus Isolated From Meat Curry Food Samples In Malaysian Restaurants," Environment & Ecosystem Science (EES), Zibeline International Publishing, vol. 4(2), pages 68-72, May.
    • Handle: RePEc:zib:zbnees:v:4:y:2020:i:2:p:68-72
    DOI: 10.26480/ees.02.2020.68.72
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    References listed on IDEAS

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    1. Marwan Msarah & Ahmed Alsier, 2018. "Protein Digestibility and Amino Acid Content of Malaysian Local Egg Pro tein Prepared by Different Methods," Environment & Ecosystem Science (EES), Zibeline International Publishing, vol. 2(1), pages 7-9, January.
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