BY MOLECULE ANCHORING TO PREPARE THE ULTRATHIN Ni LAYER WITH ELECTROCATALYTIC ACTIVITY TOWARD GLUCOSE

The preparation of ultrathin Ni layer onto the surface of glassy carbon electrode (GCE) by molecule anchoring method was studied in this paper in which two steps were involved: molecule anchoring (GCE-M) and ultrathin Ni layer adsorption (GCE-M-Ni). The result showed that by simply anchoring some molecules (purpald, bismuthiol I, DPTA, EDTA-2Na, ferrotitanium and thiourea) onto the surface of GCE, the adsorption of Ni layer would be improved relatively, thus improving the electrocatalytic ability of GCE toward sucrose, fructose and glucose. It was believed that the anchoring of these molecules helped the following adsorption of Ni layer. Good electrocatalytic characteristics for sugars oxidation of GCE-purpald-Ni were observed in this work with the immediate response, wide linear range from 0μM to 800μM and low detection limit down to 0.49μM for the sensing of total sugar (TS) concentration. The common interfering molecule such as ascorbic acid was proved of no interfering effect on the detection. Also, GCE-purpald-Ni was successfully applied to detect TS concentration in orange juice samples with high recovery rate of more than 85%. It is concluded that the molecule anchoring method can be used to the adsorption of ultrathin Ni layer onto the surface of GCE and works as one of the promising nonenzymatic sensings toward sugars.