IDEAS home Printed from https://ideas.repec.org/a/wsi/srlxxx/v25y2018i04ns0218625x18500841.html
   My bibliography  Save this article

COMPARISON OF THE IN VITRO CYTOTOXICITIES OF NITROGEN DOPED (p-TYPE) AND n-TYPE ZINC OXIDE NANOPARTICLES

Author

Listed:
  • JUNKO FUJIHARA

    (Department of Legal Medicine, Shimane University Faculty of Medicine, 89-1 Enya, Izumo, Shimane 693-8501, Japan)

  • HIDEKI HASHIMOTO

    (Department of Biomedical Sciences, Chubu University, 1200 Matsumoto, Kasugai, Aichi 487-8501, Japan)

  • NAOKI NISHIMOTO

    (Project for Advanced Technology Innovation, Shimane Institute for Industrial Technology, 1 Hokuryo, Matsue, Shimane 690-0816, Japan)

  • MIKI TONGU

    (Department of Pharmacy, Shin-Yamanote Hospital, Japan Anti-Tuberculosis Association, 3-6-1 Suwa, Higashimurayama, Tokyo 189-0021, Japan)

  • YASUHISA FUJITA

    (Interdisciplinary Graduate School of Science and Engineering, Shimane University, 1060 Nishikawatsu Matsue, Shimane 690-8504, Japan)

Abstract

The use of NPs in the health care field is increasing. Before their biological application, investigating the toxicities of both n-type ZnO nanoparticles (NPs) and nitrogen-doped (“p-type”) NPs is important. Using L929 cells, the cell viability, oxidative stress, apoptosis induction, inflammatory responses, and cellular uptake were assayed 24h after the addition of n-type ZnO NPs and nitrogen-doped NPs (which act as p-type) (25μg/mL). The ZnO NPs were fabricated using a gas evaporation method. Increased H2O2 generation and decreased levels of glutathione were more evident in with n-type than in those treated with nitrogen-doped (“p-type”) ZnO NPs. Caspase-3/-7 activity was higher in cells treated with n-type ZnO NPs than in those treated with nitrogen-doped (“p-type”) NPs. Elevated levels of TNF-α and IL-1β were observed in cell culture supernatants: IL-1β levels were higher in n-type ZnO NPs than nitrogen-doped (“p-type”) NPs. The cellular Zn uptake of n-type ZnO NPs was higher than nitrogen-doped (“p-type”) NPs. These findings show that n-type ZnO NPs have higher cytotoxicity than nitrogen-doped (“p-type”) ZnO NPs. This may be due to a reductive effect of n-type ZnO NPs that induces higher free radical production, reactive oxygen species (ROS) generation, and cellular uptake of this type of ZnO NPs.

Suggested Citation

  • Junko Fujihara & Hideki Hashimoto & Naoki Nishimoto & Miki Tongu & Yasuhisa Fujita, 2018. "COMPARISON OF THE IN VITRO CYTOTOXICITIES OF NITROGEN DOPED (p-TYPE) AND n-TYPE ZINC OXIDE NANOPARTICLES," Surface Review and Letters (SRL), World Scientific Publishing Co. Pte. Ltd., vol. 25(04), pages 1-8, June.
  • Handle: RePEc:wsi:srlxxx:v:25:y:2018:i:04:n:s0218625x18500841
    DOI: 10.1142/S0218625X18500841
    as

    Download full text from publisher

    File URL: http://www.worldscientific.com/doi/abs/10.1142/S0218625X18500841
    Download Restriction: Access to full text is restricted to subscribers

    File URL: https://libkey.io/10.1142/S0218625X18500841?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    More about this item

    Keywords

    ZnO; NPs; nitrogen-doped; n-type; toxicity; L929 cells; ROS;
    All these keywords.

    JEL classification:

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:wsi:srlxxx:v:25:y:2018:i:04:n:s0218625x18500841. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Tai Tone Lim (email available below). General contact details of provider: http://www.worldscinet.com/srl/srl.shtml .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.